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Archive > Volume 24 Issue 2 > 2017,24(2):127-133. DOI:10.3872/j.issn.1007-385X.2017.02.005 Prev Next
Basic Research
Preparation of novel nanoparticles of carboxymethyl chitosan covered with C-phycocyanin carrying CD59sp and its killing effect on HeLa cells
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Abstract:
Objective:To prepare C-phycocyanin (C-PC) contained polymeric nanaparticles with targeting ligand and study its target treatment effect on HeLa cells. Methods: The targeting drug-loading carboxymethyl chitosan (CMC) nanoparticles were synthesized by ionic-gelation method, and the particles with the most optimal condition were selected by orthogonal analysis. The surface morphology and particles size were observed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The effects of nanoparticles on the proliferation of HeLa cells were assessed by MTT assay. The tissue compatibility and safety of nanoparticles were studied by hemolysis assay. The expression of cleaved Caspase-3 and Bcl-2 was determined by Western blotting and immunofluorescent staining, respectively. Results: The optimal condition for the preparation of the nanoparticles was: CMC at 2.0 mg/ml, CaCl2 at 1.0 mg/ml, and the nanoparticles size of about 120 nm with spherical morphology; the entrapment efficiency was (62±5)%; the ratio of CMC:C-PC was 3∶1, and drug-loading amount was (20±3)%. The drug-loading nanoparticles showed slow release characteristic in vitro and without hemolysis. The nanoparticles significantly inhibited the proliferation of HeLa cells and induced the cell apoptosis; in addition, it promoted the expressions of cleaved Caspase-3 protein but suppressed the expression of Bcl-2 protein. Conclusion: The novel targeting CPC nanoparticles could inhibit HeLa cell growth, with a superior apoptosis inducing effect over the other drugs, which provided a new idea for the research on marine drugs and an important theoretical value for further study on targeting drug-loading nanoparticles.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81471546,No.81001346)
Clc Number:
