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Archive > Volume 24 Issue 2 > 2017,24(2):134-138. DOI:10.3872/j.issn.1007-385X.2017.02.006 Prev Next
Basic Research
Dendritic cells, pulsed with CD40L+ lung cancer antigens, induce homologous lung cancer immunity
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Abstract:
Objective: To explore the immunological enhancement effect of dendritic cells (DCs) derived from peripheral blood mononuclear cells (PBMCs) pulsed CD40L+ lung cancer cell antigen , on homologous lung cancer. Methods: PBMCs were isolated from peripheral blood of adults by conventional methods, then induced differentiation into DCs; recombinant CD40L+A549 cell antigen was used for DC maturation stimulation. The expressions of CD83,CD86 and HLA-DR in DCs were detected by Flow Cytometry, IL-12 secretion was detected by ELISA; the effects of CD40L+ lung cancer cell antigen pulsed DCs on proliferation of homologous lymphocyte as well as A549 cells were examined by MTT, in addition, this effect was compared to the effect of DCs that pulsed by empty vector plasmid transfected A549 cell antigen. Results: Mature DCs were successfully induced from PBMCs. The expressions of CD83, CD86 and HLA-DR in CD40L+ A549-pulsed DC group were significantly higher than those in empty group (\[4.78±1.5\]% vs \[338±1.5\]%,\[9.79±5.27\]% vs \[5.53±2.17\]% and \[11.84±8.31\]% vs \[5.37±5.48\]%，all P<0.05\]; and the secretion of IL-12 in CD40L+ A549-pulsed DC group was also higher than those in empty group and untransfected group (P<0.05). In the mean while, CD40L-pulsed DCs significantly promoted the proliferation of lymphocytes (P<0.05), and its activated homologous T cells had a significantly higher inhibitory effect on A549 cell proliferation (P<0.05). Conclusion: DC pulsed by the tumor antigens from the reconstitution CD40L transfected A549 cells could enhance its specific immunity capacity against homologous lung cancer cells.
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Project Supported:
Project supported by the Foundation of Health Bureau of Sichuan Province（No.080005）
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