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Archive > Volume 24 Issue 2 > 2017,24(2):139-144. DOI:10.3872/j.issn.1007-385X.2017.02.007 Prev Next
Basic Research
Expression of miRNA-93 and its effect on cellular biological characteristics in bladder cancer
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Abstract:
Objective:To investigate the expression of miRNA-93 and its effect on the cellular biological characteristics of bladder cancer cell line T24, and to explore the underlying mechanism.Methods:The cancer tissues and paired adjacent normal tissues from 79 patients with bladder cancer treated in Nanyang Center Hospital Affiliated to Zhengzhou University between May 2010 and May 2016 were selected for this study. The expressions of miRNA-93 were detected by Real-time PCR. After silencing of miRNA-93, the cell proliferation, apoptosis and migration were measured by CCK-8 assay, Flow cytometry and Transwell assay, respectively. The protein expressions of AKT/p-AKT and GSK3β/p-GSK3β were measured by Western blotting assay. Results: MiRNA-93 was highly expressed in both bladder cancer tissues and cancer cells (P<0.05), and its expression level was closely related to tumor size, lymph node metastasis, pathological grade stage and TNM classification (P<0.05). CCK-8 assay and cell cycle analysis showed that knockdown of miRNA-93 significantly suppressed T24 cell proliferation (P<0.05), and Flow Cytometry AnnexinV / PI double staining showed miRNA-93 silencing promoted T24 cell apoptosis (P<0.05). And Transwell assay showed that knockdown of miRNA-93 suppressed T24 cell migration. Furthermore, Western blotting showed that the protein expressions of p-AKT and p-GSK3β were significantly decreased after down-regulation of miRNA-93 (P<0.05). Conclusion:miRNA-93 could enhance cell proliferation and migration, and suppress cell apoptosis of human bladder cancer T24 cell line. And its mechanism might be related to the down-regulation of p-AKT and p-GSK3β protein expression, suggesting that miRNA-93 could be used as a potential new target for diagnostic and targeted therapy of bladder cancer.
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Project Supported:
Project supported by the Foundation of Basic Science and Fronifer Technology Research Program of Henan Province（No.142300410034）
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