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Archive > Volume 24 Issue 2 > 2017,24(2):145-150. DOI:10.3872/j.issn.1007-385X.2017.02.008 Prev Next
Basic Research
Inhibitory effects of p-hydroxylcinnamaldehyde on esophageal cancer xenograft tumor in vivo
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Abstract:
Objective: To study the inhibitory effects of p-hydroxylcinnamaldehyde（CMSP）on the proliferation of esophageal cancer cells and the growth of their xenograft tumors on BABL/c nude mouse. Methods:The inhibitory effects of CMSP on the proliferation of Kyse 30 and TE-13 esophageal cancer cells were measured by cytometry. CEA and SCC mRNA expressions in esophageal cancer cells were detected with RT-PCR assay; the expression levels of C-myc and N-myc in esophageal cancer cells were detected by Western blotting; Esophageal cancer xenograft model was constructed on nude mouse, and the mice were divided into CMSP group and control group. After experiment ended, the tumor weight and size were measured. The expression levels of C-myc and N-myc in xenografts were detected by immunohistochemical SP assay; Morphological changes of liver, spleen and tumor tissues of nude mouse were observed by H-E staining under light microscope.Results: CMSP had obvious inhibitory effect on esophageal cancer cells Kyse 30 and TE-13（\[1.7±03\] vs \[3.8±0.3\]，\[1.6±0.2\] vs \[4.5±0.4\]，P<0.01）. Compared to the control group, after CMSP treatment,(1)the expressions of CEA and SCC mRNA in Kyse 30 and TE-13 cells were inhibited (P<0.01); the expressions of C-myc and N-myc proteins were significantly inhibited (P<0.05,P<0.01); (2) the average volume and weight of xenograft tumor in CMSP group significantly decreased (P<0.05,P<001); the expressions of C-myc and N-myc proteins were decreased. No obvious morphological changes were found in liver and spleen tissues in both CMSP treatment group and control group. Conclusion: CMSP has inhibitory effects on both esophageal cancer cells in vitro and their nude mouse xenograft tumors in vivo.
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Project Supported:
Project supported by the National Natural Science Foundation for Young Scholars of China （No. 81502032）, and the Key Project of Medical Science Research in Hebei Province（No.20120120）
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