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Archive > Volume 24 Issue 2 > 2017,24(2):158-167. DOI:10.3872/j.issn.1007-385X.2017.02.010 Prev Next
Clinical Research
Expressions of CDCA8 and INCENP mRNAs in hepatocellular carcinoma and their clinical significance
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Abstract:
Objective:To explore expressions of CDCA8 and INCENP mRNAs in hepatocellular carcinoma (HCC) and their clinical significance. Methods:Illumina Nextbio microarray database was used to analyze expressions of CDCA8 and INCENP mRNAs in primary HCC and paired adjacent liver tissues. The results were verified by the RNA-Seq mRNA expression data in liver cancer database of The Cancer Genome Atlas (TCGA). Then correlations of the CDCA8 and INCENPmRNAs expressions with clinicopathological features and prognosis of the patients with HCC were analyzed combining with clinical data of TCGA. Gene sets enrichment analysis (GSEA) software was used to analyze the related pathways for high expressions of the target genes. Results: The expression levels of CDCA8 and INCENP mRNAs were significantly upregulated in HCC tissues (P<0.01). The expression level of CDCA8 mRNA was significantly correlated with histological grade, clinical stage, tumor diameter, recurrence, Eastern Cooperative Oncology Group of the United States (ECOG) grade, serum alpha fetal protein (AFP) concentration and copy number alteration (CNA) of the patients with HCC (P<0.05). The expression level of INCENP mRNA was evidently correlated with histological grade, recurrence, ECOG grade, serum AFP concentration, mutation counts and CNA of the patients with HCC (P<0.05). Overall survival (OS) and tumor free survival (TFS) of the patients with higher expression level of CDCA8 mRNA were significantly shorter than those of the patients with lower expression level of CDCA8 mRNA (P<0.01). OS of the patients with higher expression level of INCENP mRNA was significantly shorter than that of the patients with lower expression level of INCENT mRNA (P<0.05). However, there was no significant difference of TFS between the patients with higher expression level and lower expression level of INCENT mRNA. Multivariate analysis showed that the expression level of CDCA8 mRNA was an independent factor for poor prognosis of the patients with HCC (P<0.01). Analization of GSEA indicated that CDCA8 and INCENT may play cerntain roles in other biological pathways except cell cycle-related pathways. Conclusion: Expressions of CDCA8 and INCENP mRNAs appeared significant high levels in HCC tissues. CDCA8could be as an independent risk factor for prognosis of HCC and a potential novel target for therapy of the patients with HCC.
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Project Supported:
Project supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China（No.2013ZX10002010）
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