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Archive > Volume 24 Issue 3 > 2017,24(3):242-246. DOI:10.3872/j.issn.1007-385X.2017.03.005 Prev Next
Basic Research
MPPa-PDT inhibits invasion and migration of osteosarcoma MG-63 line cell
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Abstract:
Objective:To explore effect of methyl ester pyropheophorbide-a mediated photodynamic therapy (MPPa-PDT) on invasion and migration of human osteosarcoma MG-63 line cell and its possible mechanism. Methods: Using CCK8 assay, cytoactive of the MG-63 cell was detected at various time points of post-treatment with MMPa-PDT. Migration and invasion abilities of the MG-63 cell were respectively examined by Scratch and Transwell tests. Western blotting assay was used to check expression levels of E-cadherin (E-cad), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MM-9) proteins in the MG-63 cell. Results: After treatment with MPPa-PDT, proliferation ability of the MG-63 cell at 12, 24 and 48 h in MPPa-PDT group was significantly lower than those in control, MPPa and LED groups (all P<0.05), and scratch healing ability, migration activity and invasion ability of the MG-63 cell in the MPPa-PDT group were also obviously more decline than those in the other three groups (all P<0.05). Expression of E-cad protein in the MG-63 cell of the MPPa-PDT group was evidently higher than those in the MG-63 cell of the other three groups, while expressions of MMP-2 and MMP-9 proteins in the MG-63 cell of the MPPa-PDT group were lower than those in the MG-63 cell of the other three groups (all P<0.05). Conclusion: MPPa-PDT could inhibit invasion and migration abilities of the human osteosarcoma MG-63 line cell, and could up-regulate expression of E-cad and down-regulate expressions of MMP-2 and MMP-9，which could be one of the mechanisms responsible for the above inhibition action.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81572634）, and the Innovational Research Foundation for Postgraduate of the Chongqing Education Commission（No.CYS15141）
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