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Archive > Volume 24 Issue 3 > 2017,24(3):264-270. DOI:10.3872/j.issn.1007-385X.2017.03.009 Prev Next
Clinical Research
Effects of siRNA interfering expression of HMGB1 on malignant biological behaviors of bladder cancer T24 line cell and its possible mechanism
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Abstract:
Objective:To explore effects of high-mobility group box 1 (HMGB1) on proliferation, apoptosis and malignant biological behaviors of bladder cancer T24 cell line as well as its potential mechanism. Methods: Twenty cases of surgically resected bladder cancer and corresponding adjacent normal tissues form the patients who were hospitalized in Department of Urinary Surgery, the 1st Hospital affiliated to Chongqing Medical University during December 2014 to January 2016 were collected. The T24 cells were treated by RNAi technique and divided into blank control group, negative control(NC) group and interfere (siHMGB1) group. Differences of HMGB1 expression between bladder cancer and adjacent normal tissues were detected by immuno chemistry assay. CCK-8 assay, flow cytometry assay, scratch test and Transwell invasion test were used to examine effect of knocking down HMGB1 on abilities of proliferation, apoptosis, cell cycle, migration and invasion of the T24 cells respectively. Western blotting assay was used to detect expression levels of HMGB1 in BIU-87 and T24 cells and effect of knocking down HMGB1 on expression of malignant biological behavior related proteins in the T24 cells. Results: Expression of HMGB1 in the bladder cancer tissues was obviously higher than that in the adjacent normal tissues (\[67.33±4.91\] vs \[12.00±3.79\], P<0.05). Comparing with the negative control and blank control groups, proliferation of the T24 cells was inhibited in the siHMGB1 group (P<0.05). Results of flow cytometry assay suggested that apoptosis rate of the T24 cells increased and its cell cycle arrested in phase of G0/G1 after knocking down HMGB1; results of scratch and Transwell invasion tests showed that after knock-down of HMGB1, abilities of migration (P<0.05) and invasion (\[16.33±1.45\] vs \[35.00±1.53\], \[34.00±2.08\]，P<0.05\] of the T24 cells decreased. Results of Western blotting assay showed that after the knock-down, expression of E-cardherin protein was up-regulated, expressions of N-cadherin, vimentin, metrix metalloproteinase（MMP)-2, MMP-9, cyclinD1, c-Myc and β-catenin proteins were down-regulated (all P<0.05). Conclusion: HMGB1 could promote epithelial-mesenchymal transition (EMT) of the bladder cancer cells and then enhance their malignant biological behaviors. The mechanism might be mediated by β-catenin signaling pathway.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81372758）
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