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Archive > Volume 24 Issue 4 > 2017,24(4):348-354. DOI:10.3872/j.issn.1007-385X.2017.04.003 Prev Next
Monographic Report
Analysis of differentially expressed profiles of circulating tumor cells and primary tumor tissues in rectal cancer
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Abstract:
Objective:To screen specific gene of circulating tumor cells (CTCs) of rectal cancer according to the differentially expressed profile of rectal cancer CTCs and primary tumor tissues. Methods: Four cases of rectal cancer patients treated in Fujian Cancer Hospital from September 2015 to December 2016 were selected to collect tumor tissues and corresponding para-cancerous tissues, in addition, 20 ml of peripheral blood was collected. Moflo XDP cytometry was adopted to identify and sort CTCs; after purification and amplification of sample total RNA, human whole transcriptome microarray chip was used to detect the expression profiles of CTCs and tumor tissue mRNA of corresponding patients, and further to screen the CTCs specific genes. In addition, MAC3.0 bio-informatics software was used for the gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results: After sorting and testing, CTCs number sorted from each patient were 67，78，53 and 120.There were 7 755 differentially expressed gene between tumor tissues and normal para-cancerous tissues; there were 567 differentially expressed gene between CTCs and white cells; Combining the two dataset, there were 36 differentially co-expressed gene, including 16 up-regulated genes and 20 down-regulated genes (P<0.05 or P<0.01).GO function of CTCs specific genes were correlated with tumor metastasis and stem cell function, and those specific genes also participated in 22 signaling pathways related to cell migration, stem cell function and immune function. Conclusion: Compared with primary tumor gene, CTCs has a unique mRNA profile, which is the foundation of tumor recurrence and metastasis.
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Project Supported:
Project supported by the Medical Innovation Project of Fujian Province(No.2015-CXB-4) , the Natural Science Foundation of Fujian Province（No.2016J01436）, and the National Clinical Key Specialty Construction Program(No.2015-544)
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