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Archive > Volume 24 Issue 4 > 2017,24(4):355-361. DOI:10.3872/j.issn.1007-385X.2017.04.004 Prev Next
Monographic Report
Identification of circulating tumor cells and circulating tumor stem cells before and after radical resection of colorectal carcinoma by flow cytometry and its prognostic value
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Abstract:
Objective:To explore the feasibility and clinical value of indentifying circulating tumor cells (CTCs) and circulating tumor stem cells (CTSCs) by Flow cytometry before and after the radical resection of colorectal carcinoma for the prognosis prediction of colorectal cancerl (CRC) patients. Methods: Altogether 50 colorectal cancer patients without previous chemotherapeutic treatment were recruited. Approximately 15 ml peripheral blood was drawn from each patient before and one week after surgery. Mononuclear cells were isolated and divided into two groups: one was tagged with CTC surface markers (CD45, EpCAM and CK), and the other with CTSC markers (CD45, EpCAM, CD44 and CD133). After examination by Moflo XDP flow cytometry, CTC was difined as CD45-EpCAM+ CK+; and CTSC was categorized into three groups: CD44+CTSC(CD45-EpCAM+CD44+), CD133+ CTSC (CD45-EpCAM+CD133+ ) ,CD44+ CD133+ CTSC (CD45-EpCAM+CD44+CD133+ ). Results: Positive rates of pre-operative CTC and CD44+CTSC were 34% and 44%, respectively, while the positive rates of CD133+ CTSC and CD44+ CD133+ CTSC were 24% and 0%, respectively. Positive rates of post-operative CTC and CD44+CTSC were 38% and 54%, respectively, while the positive rates of CD133+ CTSC and CD44+ CD133+ CTSC were 26% and 0%, respectively. Both pre- and post-operative CTC levels were associated with the T stages of tumor (P<0.05); Moreover, post-operative CTC level had significant correlation with infiltration depth, lymphnode metastasis and distant metastasis (P<0.05). Pre-operative CD44+CTSC level was associated with tumor infiltration depth (P<0.05). Conclusion: Pre- and post-operative CTCs and preoperative CD44+CTSC all have the feasibility and practical value to be the prognostic biomarkers; Postoperative CTC positive rate may serve as a better predictive biomarker for colorectal cancer.
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Project Supported:
Project supported by Fujian Natural Science Foundation (No.2015-CXB-4)，Project supported by Fujian Natural Science Foundation（No.2016J01436），Fujian Provincial Joint Project of Health and Education Bureau (No. WKJ-FJ-14), and the National Clinical Key Specialty Construction Program(No.2013-544)
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