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Archive > Volume 24 Issue 4 > 2017,24(4):389-394. DOI:10.3872/j.issn.1007-385X.2017.04.009 Prev Next
Basic Research
Inhibition effect of siRNA silencing CXCR4 and CXCR7 on the growth of Ishikawa cell xenografts in nude mice
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Abstract:
Objective:To evaluate the effect of small interfering RNA (siRNA) of CXCR4 and CXCR7 on the growth of endometrial cancer (EC) Ishikawa cells xenografts in nude mice. Methods: EC Ishikawa cells were subcutaneously injected into the right flank of nude mice to establish animal xenograft model. When the diameter of tumors reached 5-7 mm, all tumor-bearing mice were randomly divided into five groups, and treated with intra-tumor injection of CXCR4-siRNA, CXCR7-siRNA, CXCR4-siRNA+CXCR7-siRNA, negative-siRNA, and normal saline, respectively. Growth of xenografts was observed, and the differences in volume and weight of xenografts among five groups were examined. CXCR4 and CXCR7expressions in tumor xenografts were detected by quantitative reverse transcription polymerase chain reaction (QRT-PCR), Western blotting and immunohistochemical technique, respectively. Results: Ishikawa cells xenograft model in nude mice was successfully constructed. The growth of tumor xenografts in CXCR4 siRNA group, CXCR-siRNA group and CXCR4-siRNA+CXCR7-siRNA group were significantly inhibited, compared with those in negative control and blank control group (all P<0.05). The mRNA (P<0.05) and protein (P<0.01) expressions of CXCR4 and CXCR7 were statistically down-regulated by directly injecting siRNA into xenografts. Conclusion: siRNA interfering CXCR4and CXCR7could effectively suppress the growth of human EC Ishikawa xenografts in nude mice.
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Project Supported:
Project supported by the Scientific Research Foundation of Shandong Province for Outstanding Young Scientist Award (No.BS2009SW002), and the Natural Science Foundation of Shandong Province(No.ZR2013HM012)
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