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Archive > Volume 24 Issue 4 > 2017,24(4):395-399. DOI:10.3872/j.issn.1007-385X.2017.04.010 Prev Next
Basic Research
Genistein enhances radiosensitivity of hepatoma Bel-7404 cells and its possible mechanisms
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Abstract:
Objective:To investigate the effects and mechanisms of genistein (Gen) enhancing radio-sensitivity of Hepatoma Bel-7404 cells. Methods: Bel-7404 cells were divided into four groups: normal control (NC), genistein treatment (G), radiation alone treatment (R) as well as genistein+radiation treatment group (G+R). Except the NC group, the other three groups were treated with genistein (5 μmol/L) and/or X-ray irradiation (8 Gy) at a dose rate of 300 cGy/min, respectively. The effect of Gen and x-ray on cell proliferation, cell cycle and apoptosis of Bel-7404 cells were detected using CCK-8 and flow cytometry, respectively; the expression levels of ATM-Chk2 signal pathway related proteins were detected using Western blotting.Results:The inhibitory rate on cell proliferation in G+R group was significantly higher than that in R group at 24 h after irradiation (\[16.80±2.59\]% vs \[9.76±2.11\]%, P<0.05). And the inhibitory effect was enhanced in a time-dependent manner. Compared with NC group, the cell apoptosis rate and percentage of cells in G2/M phase were all significantly increased in G, R and G+R group (P<0.05 or P<0.01). Combination of Gen with X-ray further increased cell apoptosis compared with X-ray treatment alone group (\[51.86±288\]% vs \[33.18±4.48\]%, P<0.01). Furthermore, the expressions of p-ATM and p-Chk2 were obviously up-regulated in G+R group compared with that of R, G and NC group. Conclusions: Gen could enhance radiosensitivity of hepatoma Bel-7404 cells, and the mechanism might be related with the activation of ATM-Chk2 signal pathway.
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Project Supported:
Project supported by the National Natural Science Foundation of China Grants（No. 81472770,81672350）
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