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Archive > Volume 24 Issue 5 > 2017,24(5):467-471. DOI:10.3872/j.issn.1007-385X.2017.05.002 Prev Next
Basic Research
miR-340 enhances chemotherapy resistance of colorectal cancer cells to 5-Fu by down-regulating
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Abstract:
Objective:To investigate the mechanism of coding gene of cell cyclin D1(CCND1) participating in miR-340-induced inhibition of chemotherapy resistance of colorectal cancer cells to 5-fluorouracil (5-Fu). Methods: Colorectal cancer HCT115 and SW480 cell lines were transfected with si-CCND1and miR340-mimic respectively by transient transfection technology. The sensitivity of cancer cells to 5-Fu after transfection was observed with MTT. The effect of CCND1 on miR-340 mediated suppression of chemotherapy resistance of cancer cells to 5-Fu was validated by double luciferase test. Results: After transient transfection with siCCND1 or over-expression of miR-340, the IC50 values of HCT116 and SW480 cell lines were significantly lower than that of control group \[CCND1 silencing: 10, 10 vs 20 μmol/L; miR-340 over-expressing: 20, 20 vs 40 μmol/L; all P<0.05). After co-transfection with CCND1 3′UTR wild plasmid and miR-340 inhibitor, the luciferase reporter activity in HCT 116 and SW480 cells were significantly higher than that of empty plasmid transfection group or mimic transfection group (P<0.01). Conclusion: CCND1, as an unfavorable factor, enhances the chemo-resistance of colorectal cancer cells to 5-FU by inhibiting the expression of miR-340.
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Project Supported:
Project supported by the Natural Science Foundation of Guangdong Province, China（No.2015A030311005）
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