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Archive > Volume 24 Issue 5 > 2017,24(5):478-483. DOI:10.3872/j.issn.1007-385X.2017.05.004 Prev Next
Basic Research
Crosslinked CD44 promotes apoptosis of the human mast cell leukemia HMC-1 cell via Fas pathway
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Abstract:
Objective:To investigate effect of crosslinked CD44 molecule on expression of Fas in the human mast cell leukemia HMC-1 cell line and its mediated-apoptosis of the cell. Methods: Using flow cytometry assay, expressions of the CD44 and Fas on surface of the HMC-1 cell were detected, and changes of Fas expression on the cell surface after treatment with native hyaluronan (HA) and crosslinking the CD44 molecule with anti-CD44 antibody were observed, and then effects of PKC inhibitor (H-7), PI3K inhibitor (wortmannin), inhibitors of MAPK signaling pathway, inhibitor of protein synthesis (CHX) and inhibitor of actin polymerization (cytochalasin B, CB) on expression of Fas on the surface of the cells with the crosslinked CD44 were tested. Effect of the crosslinked CD44 on expression of Fas mRNA was detected by Northern hybridization assay. With Annexin V/PI double staining, effect of the crosslinked CD44, HA or F-HA on Fas-mediated cell apoptosis was examined. Results: In the HMC-1 cell, expression of the CD44 was high and expression of Fas was low. The crosslinked CD44 molecule significantly enhanced expression of Fas on the cell surface (P<0.05), but transcription level of Fas mRNA did not obviously changed. Inhibitor of actin polymerization (CB) could inhibit up-regulation of Fas expression by the CD44 (P<0.05). Inhibitors of cell signaling pathway and protein synthesis which were detected couldn’t inhibit up-regulation of Fas expression (P<0.05). All of F-HA and the crosslinked CD44 could promote Fas-mediated cell apoptosis. Conclusion: The crossliking of CD44 up-regulates Fas expression in the HMC-1 cell, and enhances Fas-mediated cell apoptosis. The CD44 molecule could become a novel target for treatment of the mast cell leukemia.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81402228)，the Hebei Province Natural Science Foundation (No.H2015206216)，the Hebei Province Medical Foundation (No.ZL20140334)，and the Hebei Province Education Foundation (No.QN2014049)
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