Mobile website
Archive > Volume 24 Issue 5 > 2017,24(5):484-489. DOI:10.3872/j.issn.1007-385X.2017.05.005 Prev Next
Basic Research
Effect of CHD1L gene silencing on malignant biologic behaviors of prostate cancer PC3 cell line and its possible mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To explore the influence of CHD1L gene (chromodomain helicase/ATPase DNA binding protein 1-like gene) on the invasion and migration ability of prostate cancer cells and the potential mechanisms. Methods: Real-time fluorescent quantitative-PCR assay was carried out to detect mRNA expression of CHD1L gene in prostate cancer cell lines LNCAP, PC3 and DU 145 as well as normal prostate epithelial cell line RWPE-1. siRNA transfection was used to deplete CHD1L expression in PC3 cells. Then Transwell invasion assay and wound healing test were employed to detect the effect of CHD1Lsilencing on the invasion and migration ability of PC3 cells. Protein expressions of MMP-9, N-cadherin and E-cadherin were examined by Western blotting assay. Results: Compared to normal prostate epithelial cells, three prostate cancer cell lines all showed significantly higher expression of CHD1L mRNA (P<0.01), and PC3 cells demonstrated the highest CHD1LmRNA expression. Invasion assay showed that the trans-membrane cell number in CHD1L silencing group was significantly lower than that in negative and blank control groups (49.67±6.67 vs 113.67±5.69 and 112.00±12.49, P<0.05). Wound healing test also exhibited lower healing degree in CHD1L silencing group than that in negative and blank control groups at 48 h (\[21.27±3.27\]% vs \[48.47±5.72\]% and \[49.93±3.35\]%, respectively, P<0.05). Furthermore, protein expressions of MMP-9 and N-cadherin decreased while E-cadherin increased in CHD1L gene depletion cells. Conclusions: CHD1L silencing could dampen the invasion and migration ability of prostate cancer PC3 cell line, and this might be accomplished by regulating MMP-9 and EMT related proteins.
Keywords:
Project Supported:
Project supported by the Key Task Project in Medical Science Research Program of Henan Province（No. 201303155）
Clc Number:
