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Archive > Volume 24 Issue 5 > 2017,24(5):490-496. DOI:10.3872/j.issn.1007-385X.2017.05.006 Prev Next
Basic Research
Effects of EZH2 and H3K27me3 expression on migration and invasion of esophageal carcinoma cells
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Abstract:
Objective:To study the effect of enhancer of zeste homolog 2(EZH2) and histone H3 methylated Lys27 (H3K27me3) on the migration and invasion ability of esophageal squamous cell cancer (ESCC) cells by transfection of vectors expressing/knock down EZH2. Methods: Quantificational real-time PCR(qRT-PCR) and Western blotting were performed to evaluate the expression levels of EZH2in esophageal carcinoma cell lines (KYSE30, KYSE170, TE1, Eca109), as well as the effect of over-expression or knockdown of EZH2 on H3K27me3 expression in ESCC cell lines. Effect of EZH2 over-expression or knockdown on migration and invasion abilities of ESCC cells were examined by wound healing assay and Transwell invasion test, respectively. The expression level of MMPs in ESCC cells after over-expression or knockdown of EZH2 were detected by qRT-PCR.Results: The expressions of EZH2 and H3K27me3 in Eca109 and TE1 cells were significantly higher than those in KYSE30 and KYSE170 cells (P<0.05). Over-expression of EZH2 promoted the expression of H3K27me3 in KYSE30 and KYSE170 cell lines (P<0.05). Knockdown of EZH2inhibited the expression of H3K27me3 in Eca109 and TE1 cell lines (P<0.05). The trans-membrane number of KYSE30 and KYSE170 cells was significantly increased (\[281.33±4.10\] vs \[132.00±4.00\], P<0.05; \[241.67±4.04\] vs \[105.33±3.51\], P<0.05), and the migration distance of KYSE30 and KYSE170 cells was significantly increased (\[63.6±1.2\] vs \[23.0±2.3\] μm, P<0.05; \[62.5±2.5\] vs \[21.2±1.0\] μm, P<0.05) after over-expression of EZH2. Knockdown of EZH2significantly decreased the trans-membrane number of Eca109 and TE1 cells (all P<0.05), and the migration distance was also decreased after transfection with shEZH2 (all P<0.05). Conclusion: EZH2 can enhance trimethylation of lysine 27 on histone H3 (H3K27) of the target gene promoters, and promote the migration and invasion abilities of esophageal carcinoma cell lines.
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Project Supported:
Project supported by the Science and Technology Supporting Program of Hebei Province（No. 14277732D，No.152777184)
Clc Number:
