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Archive > Volume 24 Issue 5 > 2017,24(5):497-502. DOI:10.3872/j.issn.1007-385X.2017.05.007 Prev Next
Basic Research
Effect of lncRNA HOXA11-AS on proliferation,migration and invasion of osteosarcoma cell lines
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Abstract:
Objective:To investigate the expression of lncRNA HOXA11-AS in osteosarcoma, and to explore its effect on proliferation, migration and invasion of osteosarcoma cells and the possible mechanism. Methods:Thirteen cases of osteosarcoma tissues and corresponding adjacent normal tissues were collected from the Department of Bone Surgery, the People’s Hospital of Zhejiang Province during January 2012 to December 2015. Osteosarcoma cell MG63, U205 lines and human osteoblast cell hFOB1.19 line were used. Real-time fluorescent quantitative PCR was performed to detect the lncRNA HOXA11-AS expression in osteosarcoma specimens and osteosarcoma MG63 and U20S cell lines. Lentiviral vectors that stably over-expressing lncRNA HOXA11-AS were constructed and transfected into MG63 and U20S cell lines; CCK8 assay and colony-formation assay were performed to investigate the effect of lncRNA HOXA11-AS on osteosarcoma cell proliferation; Transwell assay was performed to investigate the effect of lncRNA HOXA11-AS on migration and invasion of osteosarcoma cells. Nude mice model of MG63 osteosarcoma xenograft, which over-expressing lncRNA HOXA11-AS, was established to observe the effect of lncRNA HOXA11-AS on xenograft growth in mice, by comparing with Lv-NC transfected cell xenografts. Results: lncRNA HOXA11-AS was markedly down-regulated in tumor tissues and osteosarcoma cell lines (P<0.01). Over-expression of lncRNA HOXA11-AS significantly suppressed (1) the proliferation, migration and invasion of U20S and MG63 cells (proliferation: \[4.03±0.98\] times vs \[6.96±0.54\] times, \[4.68±0.77\] times vs \[8.87±1.23\] times, all P<0.01; migration: \[83.00±6.03\] vs \[168±12.54\], \[96±8.77\] vs \[184±14.63\], all P<0.01; invasion: \[35.00±3.48\] vs \[97.00±8.32\], \[38.00±1.73\] vs \[87.00±6.37\], all P＜0.01); (2) the growth of xenografts in rats（P<0.01）. Conclusion:lncRNA HOXA11-AS significantly down-regulated in osteosarcoma cells, and suppressed the progression of osteosarcoma. lncRNA HOXA11-AS can be served as a therapeutic target for osteosarcoma.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81672769)
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