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Archive > Volume 24 Issue 5 > 2017,24(5):510-515. DOI:10.3872/j.issn.1007-385X.2017.05.009 Prev Next
Basic Research
Effect of rNDV IL-29 on malignant biological behavior of lung adenocarcinoma A549 cells
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Abstract:
Objective: To investigate the effects of recombinant Newcastle disease virus (rNDV) expressing IL-29（rNDV IL-29）on the proliferation, migration and apoptosis of lung adenocarcinoma A549 cells. Methods: A549 cells were treated with rNDV IL-29, NDV and PBS respectively, namely rNDV IL-29 group, NDV group and control group. The protein and gene expression of IL-29 in A549 cells were examined by RT-PCR, immunofluorescence and Western blotting; MTT assay, colony formation assay and scratch assay were used to evaluate the effect of rNDV IL-29 on proliferation and migration ability of A549 cells; Flow cytometry and Western blotting were used to examine the effect of rNDV IL-29 on apoptosis of A549 cells. Results:Compared with NDV group and control group, (1) IL-29 protein and gene expressions were dramatically increased in rNDV IL-29 group. (2) the rate of cell proliferation in rNDV IL-29 group was significantly inhibited (\[56.48±11.89\]% vs \[42.18±6.18\]%, P<0.05), and migration ability of A549 cells in rNDV IL-29 group was statistically decreased (\[5.00±4.00\]% vs \[32.43±3.71\]%, \[84.57±3.96\]%, all P<0.05). (3) the expression of caspase3 in rNDV IL-29 group was significantly up-regulated (\[29.67±1.53\]% vs \[18.33±3.06\]%, \[7.0±6.08\]%, all P<0.05), while Bcl-2/bax level was remarkably reduced (\[12.00±2.65\]% vs \[42.33±586\]%, \[67.00±6.25\]%, all P<0.05\]. Conclusion: rNDV IL-29 inhibits the proliferation and migration of A549 cells and promotes apoptosis of A549, suggesting that rNDV IL-29 may be served as a potential anticancer drug.
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Project Supported:
Project supported by the Natural Science Foundation of Jiangsu Province(No.BK20151333)
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