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Archive > Volume 24 Issue 5 > 2017,24(5):516-520. DOI:10.3872/j.issn.1007-385X.2017.05.010 Prev Next
Basic Research
IL-17A promotes migration and invasion of colon cancer SW480 cells through NF-κB pathway mediating MMP2/9 expression
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Abstract:
Objective:To explore the effect and mechanism of IL-17A on invasion and migration of colon cancer SW480 cells.Methods: Colon cancer SW480 cells were in vitro cultured and divided into experimental group (IL-17A 50 ng/ml) and control group (blank group). The migration ability was observed by cell scratch assay, the invasion feature of SW480 cells was detected by Transwell assay, and the expressions of MMP-2/9 protein and PI3k/AKT/NF-κ B pathway related proteins were tested by Western blotting. Results: After the treatment with IL-17A(50 ng/ml), (1) the migration distance and trans-membrane number of SW480 cells significantly increased (\[2.49±018\] mm vs \[1.54±0.21\] mm; \[262.00±24.60\] vs \[92.00±31.16\]; all P<0.05); (2) the expression of MMP-2/9 protein in SW480 cells was obviously up-regulated (\[0.41±0.05\] vs \[0.23±0.03\]; \[0.76±0.09\] vs \[0.25±0.04\], all P<0.05); (3) AKT-phosphorylation level in SW480 cells was significantly increased (\[0.72±0.1\] vs \[028±0.04\], P<0.05), and the protein expressions of P65 and P50 were significantly increased (\[0.78±0.10\] vs \[0.35±0.04\]; \[0.85±0.15\] vs \[0.44±0.06\], all P<0.05); however, the protein expressions of c-Rel, ReLB, and P52 showed no significant difference (P>0.05). Conclusion: IL-17A induced the migration and invasion of colon cancer SW480 cells, and increased tumor cell viability; the mechanism may be related to the activation of PI3K/AKT/NF-κB pathway and up-regulation of MMP-2/9 protein expression.
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Project Supported:
Project supported by the General Science and Technology Project from the Education Bureau of Liaoning Province（No.L2014333）
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