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Archive > Volume 24 Issue 5 > 2017,24(5):527-532. DOI:10.3872/j.issn.1007-385X.2017.05.012 Prev Next
Clinical Research
Biomarkers of lung carcinoma for evalution of the immunocytotherapy selected by solid phase antibody chip against multiple factors
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Abstract:
Objective:Applying solid phase antibody chip against multiple factors to screen serum marker proteins of the patients with lung carcinoma who were treated by immunocytotherapy as clinical diagnosis index and to establish an evaluation model for clinical efficacy of the patients with lung carcinoma.Methods:Using Proteome ProfilerTM solid phase antibody chip against multiple factors and Image J software to analyze gray scale/optical density of the serum marker proteins from 5 patients with advanced lung carcinoma, 5 patients with improved lung carcinoma and 10 healthy individuals. The protein gray values obtained were analyzed by SPSS software, and Fisher model for evaluation of clinical efficacy of the patients with lung carcinoma was established. Results: Analyzing and comparing the common marker proteins among the patients with advanced lung carcinoma, the patients with improved lung carcinoma and the healthy individuals, 8 proteins that are dipeptidyl peptidase Ⅳ, growth hormone, IL-4, myeloperoxidase, osteopontin, receptor of advanced glycation endproducts, TNF-α, and urokinase type plasminogen activator receptor with significant difference (P<0.05) were obtained. Clustering and analyzing data for all of the patients and the healthy individuals in the experiment found that the 8 proteins can very well distinguish the patients with advanced lung carcinoma from the patients with improved lung carcinoma and the healthy individuals. The Fisher model for lung carcinoma was confirmed. Conclusion: The solid phase antibody chip against multiple factors and optimal statistical methods could screen out the serum protein biomarkers related to occurrence, development and efficacy of lung carcinoma, which could establish a certain basis of clinical trail for researches on the mechanism of lung carcinogenesis as well as clinical diagnosis and treatment of the lung carcinoma. It could play an important guiding role in the individualized treatment of the lung carcinoma.
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Project Supported:
Project supported by the Foundation of Liaoning Education Department(No. 201610163L28)
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