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Archive > Volume 24 Issue 5 > 2017,24(5):533-537. DOI:10.3872/j.issn.1007-385X.2017.05.013 Prev Next
Clinical Research
Connexin 43 enhances invasion capacity of bladder cancer cells through P38/MAPK signaling pathway
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Abstract:
Objective:To explore the effect of connexin 43(Cx43) on invasion ability of bladder cancer cells and the possible mechanisms. Methods: Fifty-two bladder cancer tissues and 32 precancerous tissues resected from June 2014 to September 2015 at the Department of Urology of the Affiliated Hospital of Chengde Medical University, as well as human bladder cancer 5637 cell line were collected for this study. Immunohistochemical method was used to detect the expression of Cx43 protein in bladder cancer tissues. Cx43 liposome plasmid (Transfection group), empty liposome plasmid (Transfection control group), siRNA (siRNA group) and siRNA control (siRNA control gorup) were transfected into bladder cancer 5637 cells. The efficiency of over-expression and knockdown was evaluated by Western blotting; the changes in invasion of bladder cancer cells were evaluated by Transwell assay. The changes in protein expressions of MMP-2, MMP-9, P38 and P-P38 were detected by Western blotting. Results: The expression of Cx43 protein in bladder cancer tissues was significantly higher than that in paracancerous tissues (\[5.21±0.33\] vs \[2.84±0.19\], P<0.01). Transfection of Cx43 liposome or siRNA successfully up-/down-regulated the expression of Cx43 in 5637 cells. Compared with control group, the migration ability of 5637 cells in Cx43 over-expression group was increased significantly (\[1.36±0.04\] vs \[0.70±0.15\]，P<0.01), while that of siRNA group was decreased significantly (\[0.20±0.08\] vs \[0.59±0.13\], P<0.05). The protein expressions of MMP-2, MMP-9, P-P38 and P38 in Cx43 liposome transfection group were significantly higher than those in control group (P<0.01 or P<0.05), while the expressions in siRNA group were significantly lower than those in the control group (all P<0.01). Conclusion: Cx43 could enhance invasion ability of bladder cancer cells, and the mechanism might be related with the activation of P38/MAPK signaling pathway.
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Project Supported:
Projct supported by the Science and Technology Research and Development Projects of Chengde City（No.201422014）, and the Scienific Research Fundation for Youths of Hospital Affiliated to Chengde Medical College （No.201405）
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