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Archive > Volume 24 Issue 6 > 2017,24(6):615-619. DOI:10.3872/j.issn.1007-385X.2017.06.007 Prev Next
Basic Research
Ginsenoside Rg3 promotes the apoptosis of breast cancer MDAMB231 cells via regulation of mammaglobinA expression
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Abstract:
Objective:To study the mechanism of ginsenoside Rg3 inhibiting the proliferation of breast cancer MDAMB231 cells via regulation of mammaglobinA (MGBA) expression. Methods: MTT assay and flow cytometry was used to detect the effect of different concentrations (5,10,15 μg/ml) of Rg3 on the proliferation and apoptosis of breast cancer cells; Western blotting was used to detect the expression of MGBA in breast cancer cells. MDAMB231 cells were treated with Rg3 and/or siRNAMGBA; MTT and Flow cytometry were used to detect their effect on the proliferation and apoptosis of treated cells while Western blotting was used to detect the expression of MGBA in cells; sulfur electrode assay was used to detect H2S secretion in breast cancer MDAMB231 cells. Results:48 h after cell treatment, the inhibitory rates on proliferation of MDAMB231 cells in Rg3 groups (5,10,15 μg/ml) were significantly higher than that of control group (\[18.78±0.82\]%, \[33.25±1.17\]%, \[35.11±0.94\]% vs \[9.72±0.91\]%, all P<0.05\]; Rg3 promoted the apoptosis of MDAMB231 cells (P<0.05), and significantly enhanced the expression of MGBA protein in MDAMB231 cells (P<0.05); Compared with the control group, the inhibitory rate on proliferation of MDAMB231 cells in Rg3 group was significantly increased (\[30.12±1.01)% vs (10.66±0.59)%, P<0.05\], while the inhibitory rates in Rg3+siRNAMGBA and siRNAMGBA group were significantly reduced (\[6.61±0.63\]%, \[702±0.46\]% vs \[1066±0.59\]%, all P<0.05); The expression of MGBA and cystathionineγ lyase (CSE) as well as the secretion of H2S in Rg3 group were significantly enhanced; however, their expressions in Rg3 + siRNAMGBA group and siRNAMGBA group were significantly inhibited（all P<0.05）. Conclusion: Rg3 can significantly inhibit the growth of MDAMB231 breast cancer cells and promote its apoptosis, and this effect may be realized by enhancing MGBA expression and activating H2S/CSE system.
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Project Supported:
Project support by Natural Science Foundation of Liaoning Province（No.2014022044）
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