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Archive > Volume 24 Issue 6 > 2017,24(6):670-674. DOI:10.3872/j.issn.1007-385X.2017.06.017 Prev Next
Technigue and Method
WAVE bioreactors for cytokine induced killer cell culure
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Abstract:
Objective:To investigate the efficacy of WAVE bioreactorin the culture and amplification of cytokine induced killer (CIK) cells and their killing activity on tumor cells. Methods: Peripheral blood mononuclear cells (PBMCs) from 8 cancer patients were isolated and cultured by using traditional CIK amplification method (CIK group) and WAVE bioreactors (WAVE group)respectively. Trypan blue staining was used for cell counting, and Flow cytomery was used to comparethe amplification efficiency, functionality and subtype compositionbetween two groups; K562 cell, as the target cell, was used to detect the killing activity of amplified CIK cells of two methods. Results: The proliferated number of CIK cells in WAVE groupwassignificantly higher than that of CIK group(P<0.05 or P<0.01); the proportions of CD3+CD8+ cells and CD3+CD56+ cells in WAVE group were significantly higher than those in CIK group on Day 14 (\[78.56±2.99\]% vs \[74.54±3.02\]%, \[48.33±7.01\]% vs \[40.69±6.43\]%, all P<0.05); However, the proportion of Tregs cell was significantly decreased (P<0.05) in WAVE group. Moreover, it was observed that CIK cells culturedin the WAVE bioreactor group displayed a significantly higher cytotoxic capacitythan that in CIK group at the E/T ratio of 10∶1 and 20∶1, and the proportion of CD3+CD8+CD107a+ in CIK cells of WAVE group was significantly higher （\[29.43±4.97\]% vs \[25.19±4.91\]%, P<0.05). Conclusion: WAVE bioreactor system could produce more CIK cells with high purity, andCD3+CD8+ cells and CD3+CD56+NKT cells account higher proportion; CIK cells amplified by WAVE bioreactor exhibited higher killing effect on tumor K562 cells compared to the CIK cells amplified by traditional culture technique.
Keywords:
Project Supported:
Project supported by the Special Fund for the Development of Scientific Research in Liaoning Province（No.LNZC20141101146），and the Doctoral Scientific Research Foundation of Liaoning province (No.201601412).
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