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Archive > Volume 24 Issue 7 > 2017,24(7):700-707. DOI:10.3872/j.issn.1007-385X.2017.07.002 Prev Next
Monographic Report
Effect of lncRNAXLOC_005009 on biological properties of esophageal cancer cell
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Abstract:
Objective: To explore effect of long non coding RNA (lonRNA) of XLOC_005009 gene on biological properties, proliferation, migration, invasion, cell cycle and apoptosis in vitro, of esophageal cancer cell. Methods:The surgically resected cancer and para-cancerous tissues of 25 patients with esophageal cancer who hospitalized in the Tumor Institute of the 4 th Hospital of Hebei Medical University during 2015 to 2016 were collected. Expressions of XLOC_005009 gene in human esophageal cancer Eca109 and Kyse170 line cells, the esophageal cancer and para- cancerous tissues were detected by RT-PCR. pcDNA3.1-XLOC_005009 over-expression plasmid was structured and transfected into the Eca109 and Kyse170 cells. MTS , colony forming, scratching, Transwell chamber and flow cytometry assays were used respectively to check proliferation, cloning efficiency, migration, invasion, cell cycle and apoptosis of the cells, before and after transfection of the over-expression plasmid. Results: Expression of XLOC_005009 mRNA in the esophageal cancer tissue was obviously lower than that in the para-cancerous normal tissue (0.06±0.06 vs 0.21±0.19, P＜0.05), and expressions of XLOC_005009 mRNA in the esophageal cancer cells were all lower than that in the control group. In the Eca109 and Kyse170 cells transfected with the over-expression plasmid, expressions of XLOC_005009 gene were higher than those in the control groups (Eca109 cell: 039±0.17 vs 0.02±0.00; Kyse170 cell: 0.35±0.08 vs 0.01±0.01, all P＜0.05). And comparing with the control group, proliferation abilities of the Eca109 and Kyse170 cells with over-expression of XLOC_005009 gene were significantly weakened (P<0.05), their cloning efficiency evidently reduced, transmembrane cell numbers of the cells remarkably decreased (Eca109 cell: 146.40±34.47 vs 193.00±26.33; Kyse170 cell:157.80±32.51 vs 269.00±29.89, all P<0.05), migration efficiency of the Eca109 cell didn’t markly change, but that of the Kyse170 cell obviously reduced; S phase cell ra- tio of the cells increased; effecting on apoptosis of the cells was not obvious. Conclusion: Low-expression of XLOC_005009 might be closely related to occurrence and development of the esophageal cancer. Over-expression of XLOC_005009 could inhibit proliferation, invasion and migration in vitro of the esophageal cancer cells.
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Project Supported:
Project supported by the National Natural Science Foundation (No. 81472335), and the Natural Science Foundation of Hebei Province (No. H2015206196)
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