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Archive > Volume 24 Issue 7 > 2017,24(7):727-732. DOI:10.3872/j.issn.1007-385X.2017.07.006 Prev Next
Basic Research
Effect of lncRNA FENDRR on proliferation, migration and invasion of the Xuan-wei lung carcinoma XWLC-05 cell
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Abstract:
Objective: To detect expressions of FENDRR long non coding RNA(lncRNA) in the lung adenocarcino-ma XWLC-05 cell line from the Xuanwei area and in the non small cell lung cancer (NSCLC) A549 cell line, and to explore its effect on proliferation, migration and invasion of the cell lines. Methods: Expressions of FENDRR gene in the XWLC-05 and the A549 cells were detected by Real-time quantitative fluorescence PCR. Over-expression plasmid pEX-3-FENDRR and lentiviral vector LV3-FENDRR were structured, then respectively transfected into theXWLC-05 and the A549 cells. After the transfection, expressions of FENDRR in the XWLC-05 and the A549 cells were tested by real-time quantitative fluorescence PCR. MTS assay was used to examine proliferation of the cells and Transwell assay used to test migration and invasion abilities of the cells. Results: Expression of ENDRRgene in the XWLC-05cell was obviously lower than that in the A549 cell. Level of FENDRR mRNA in the XWLC- 05cell transfected with pEX-3-FENDRR was enhanced and, which in the A549 cell transfected with LV3-FENDRR reduced. Comparing with the control group, over-expression of FENDRR could significantly inhibit proliferation of the XWLC-05cell ([1.23±0.13] vs [1.46±0.25], P<0.05), and reduce its abilities of migration and invasion (P< 0.05). Interference of FENDRR expression could promote proliferation ([0.85±0.02] vs [0.77±0.08], P< 0.05), mi- gration and invasion of the A549 cell. Conclusion: Down-regulation of lncRNA FENDRR expression could related with occurrence and development of the uanweilung adenocarcinoma, which could play a role of tumor suppres-sion in the process of tumor malignization and inhibit proliferation and metastasis of the tumor cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81460064, No. 81460325), the Science and Technology Department of Yunnan Province-Kunming Medical University Joint Special Application Basic Research Foundation （No.2015FB047）and the Leading Talent Plan of the Health System in Yunnan Province (L-201202)
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