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Archive > Volume 24 Issue 7 > 2017,24(7):756-761. DOI:10.3872/j.issn.1007-385X.2017.07.010 Prev Next
Basic Research
Immuno-inhibitory effect of DC transfected with MUC1 gene on xenograft tumor of the breast carcinoma MCF7 cell in nude mouse
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Abstract:
Objective: To explore inhibitory effect of dentritic cell (DC) transfected with mucin 1 (MUC1) gene on xenograft tumor of human breast carcinoma MCF7 cell in nude mouse. Methods: DC of healthy adults were in-duced-cultured in vitro. Plasmid with human pcDNA3.1-MUC1 gene were transfected into the DC by a lipofection assay. ELISA assay was used to detect secretion ability of cytokines IL-12 and TNF-α by the transfected DC. LDH release assay was used to exam killing activity of specific cytotoxic T lymphocytes (CTL) induced by the transfect-ed DC to the breast carcinoma MCF7 cell. The xenograft tumors of human breast carcinoma MCF7 cell in nude mice were treated by the DC transfected with MUC1 gene, the DC transfected with empty plasmid and normal sa-line in subcutaneous injection, and growth inhibition of the xenograft tumors in nude mice were observed. Results:Secretion abilities of IL-12 and TNF-α by the DC transfected with pcDNA3.1-MUC1 gene were obviously more en-hanced than those by the DC transfected with empty plasmid ( IL-12:[202.52±29.61] vs [10.83±1.02] pg/ml; TNF-α:[349.07±79.42] vs [9.26±1.52] pg/ml, all P<0.01). Killing activity of the specific CTL induced by the DC transfect-ed with pcDNA3.1-MUC1 genewas more obvious than that by the CTL of the control group, and the killing rates at effector/targetor ratio of 10:1, 5:1 and 2.5:1 were 56.2%, 38.9% and 25.8% respectively (all P<0.01). Inhibitory ef-fect of the DC transfected with MUC1 gene on the xenograft tumors of breast carcinoma MCF7 cell in nude mice was significantly stronger than that of the DC transfected with empty plasmid (P<0.05). Conclusion: The DC trans-fected with MUC1 gene might induce specific CTL, which might have stronger anti-tumor immuno effect on the breast carcinoma MCF7 cell.
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Project Supported:
Project supported by the Scientific Research Foundation for Projects of Dalian City's Health and Family Plaming Commission (No.2012-0407L)
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