Mobile website
Archive > Volume 24 Issue 7 > 2017,24(7):762-766. DOI:10.3872/j.issn.1007-385X.2017.07.011 Prev Next
Clinical Research
Research on clinicopathological relationship of SNP of PD-L1 gene 3’UTR with BUC by case-control studies
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To explore relationship between single nucleotide polymorphism (SNP) of programmed death ligand 1 (PD-L1) gene, an immune checkpoint molecule,3’untranslated region (3’UTR) and onset risk, clini-cal pathological features of bladder urothelial carcinoma (BUC). Methods: A case-control study was used. Poly-merase chain reaction-ligase detection reaction (PCR-LDR) assay was used to detect the genotype distribution fre-quency of rs4143815 and rs2297136 locus in PD-L1 gene 3’UTR of the 213 patients with BUC who were hospital-ized in Department of Urology Surgery, the Affiliated Hospital of Qingdao University for surgical treatment during June 2013 to December 2015 and the 251 individuals for health examination during the same stage. A relationship between different genotypes and onset risk of BUC as well as clinicopathological features of the patients with BUC was analyzed by chi-square test and unconditionalmultivariate Logistic regression assay. Results: Significant differ-ences of genotype frequencies at the rs4143815loci were found between BUC cases and controls. Onset risk of BUC in the individuals carring GG genotype was 2.83 times (95%CI: 1.82-4.64, P<0.01) of the individuals carring CC genotype and onset risk of BUC in the individuals carring G mutation gene (CG/GG genotype) was 1.53 times (95% CI:1.01-2.24, P<0.01) of the individuals carring CC genotype. Furthermore in the BUC group, frequency of carring G mutation gene at the rs4143815loci was significantly correlated to pathological grade of BUC and clinical staging of the patients with BUC (P<0.05，P<0.01). However at the rs2297136 loci, any significant difference of gnotype distribution frequency between the BUC group and the control group was not found. Onset risks of BUC among the individuals carring CC, CT and TT genotypes were not obviously different. Conclusion: SNP of rs4143815loci in PD-L1 gene 3’UTR could closely related to onset rick and malignant progression of BUC.
Keywords:
Project Supported:
Project supported by the Na-tional Natural Science Foundation of China (No. 81101932), and theApplied Basic Research Foundation of Qingdao City (No. 15-9-1-105-jch）
Clc Number:
