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Archive > Volume 24 Issue 8 > 2017,24(8):815-827. DOI:10.3872/j.issn.1007-385X.2017.08.001 Prev Next
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Principles and applications of genome-editing technologies in cancer research
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Abstract:
Genome-editing technologies that enable precise and stable modification of the genome are sparking a revolution in life science research and disease management. The discovery of the CRISPR/Cas9 technology repre-sents a huge breakthrough in the field of genome editing due to its easy operation and high efficiency, empowering scientists to manipulate genome editing to a broader extension. In this review, the classification of genome-editing technologies, the potential applications of the CRISPR/Cas9 system (including gene knockout, knock-in, methods of delivering CRISPR/Cas9 into cells, enhancing specificity of the CRISPR/Cas9, and accurate regulation of the CRIS-PR/Cas9) as well as broader applications of the CRISPR/Cas9 system (including base replacement, epigenetic regu-lation, regulation of endogenous gene expression, and genome-wide genetic screening) will be elaborated, respec-tively. Moreover, CRISPR/Cas9 in the field of cancer research, including generation of mouse models of cancer, gen-eration of cancer models of chromosome rearrangement, high-throughput screening of genes associated with tumor metastasis, as well as treatment of cancer will also be illustrated, providing readers with a general understanding of the CRISPR/Cas9 technology.
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Project Supported:
Projectsupported by the National Natural Science Foundation of China（No.81370302）
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