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Archive > Volume 24 Issue 8 > 2017,24(8):833-837. DOI:10.3872/j.issn.1007-385X.2017.08.003 Prev Next
Prompt Report
siRNA targeting decoy receptor 3 gene increases the radiosensitivity of human pancreatic cancer cells
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Abstract:
Objective: To investigate the effect of siRNA targeting decoy receptor 3 gene on the radiosensitivity of pancreatic cancer cells and its related mechanism. Methods: Plasmid stably expressing DcR3 siRNA sequence was constructed and transfected into pancreatic cancer AsPC-1 cell line by liposome; control group, siRNA(-) negative control group and DcR3 siRNA group were set up. Then DcR3 expression in AsPC-1 cells was detected by Western blotting. Effect of DcR3 siRNA transfection on radiation sensitivity of AsPC-1 cells was detected by plate clone for-mation assay. Cell apoptosis was analyzed by Flow cytometry; the expressions of Caspase-8, Caspase-3 and PARP-1 were detected by Western blotting and RT-PCR after transfecting with DcR3 siRNA. Results: The expression of DcR3 protein in DcR3 siRNA group was significantly lower than that in control or siRNA(-) group (P<0.01). The colony formation rate of DcR3 siRNA group was significantly lower than that in control group and siRNA(-) group (P<0.01), and the survival fraction(SF) value of DcR3 siRNA group was decreased and the ratio of α/β was in-creased (P<0.01). The cell apoptosis rate of DcR3 siRNA group was significantly higher than that of control group or siRNA(-) group (P<0.01). DcR3 siRNA could significantly up-regulate the expression of Caspase-8 and Caspase-3 and down-egulatetheexpressionofPARP-.Conclusion:siRNAsilencingDcR3genecanincreasetheradiosensitiv-ityofpancreaticcancercellsbyactivatingthe apoptosis factors Caspase-8 and Caspase-3, promoting cell apoptosis.
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Project Supported:
Project supported by the Natural Science Foundation of Hunan Province(No. 14JJ3136), and the Research Fundation of Chenzhou Science and Technology Bureau (No. CZ2013096)
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