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Archive > Volume 24 Issue 8 > 2017,24(8):838-844. DOI:10.3872/j.issn.1007-385X.2017.08.004 Prev Next
Basic Research
Effect of microRNA-150 on the radiosensitivity of NK/T cell lymphoma tissues and NK-92 cells
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Abstract:
Objective: To investigate the expression of MicroRNA-150 (miR-150) in NK/T cell lymphoma tissues and its effect on radio-sensitivity of NK-92 cells. Methods: Thirty-six patients with NK/T cell lynphoma that treat-ed in Zhujiang Hospital Affiliated to Southern Medical University were included as study subjects, and their tissue samples were collected. All the patients received similar radiotherapy, and the short-term efficacy was evaluated by the standard of International Workgroup (IWG) of Lymphoma. The patients were further divided into CR (complete remission) group and Non-CR (non-complete remission) group according the treatment efficacy. The miR-150 ex-pression in lymphoma tissues and NK/T cell lymphoma cell lines were detected by qRT-PCR. The NK-92 cells were then transfected with miR-150 mimics. The effect of miR-150 mimics on NK-92 cell radiosensitivity was analyzed by MTT and colony formation assay; effect of miR-150 mimics on radiation induced apoptosis of NK-92 cells were analyzed by Flow Cytometry; and the effect of miR-150 mimics on the expression of apoptosis-related proteins (Cas-pase 3 and PARP) in NK-92 cells was determined by Western blotting. Results: According to IWG criteria, 12 pa-tients had CR while the other 24 patients had Non-CR. Compared with CR group, the microRNA-150 level in Non-CR group was significantly decreased(P<0.05). Compared with the normal sCD3 - CD56 + NK cells, miR-150 was sig-nificantly lower in NK/T cell lymphoma tissues (9.10 ±0.19 vs 4.01± 0.22 P<0.01 ) and five NK/T cell lymphoma cell lines (P<0.05); over-expression of miR-150 significantly decreased the proliferation and colony formation of NK-92 cells (all P<0.01), and increased the sensitivity enhancement ratio (SER) after radiation (the SER was 5.375 after 10 Gy exposure; in addition, over-expressions of miR-150 significantly promoted the radiation-induced apopto-sis of NK-92 cells [(37.3±1.24)% vs (28.3±2.34)%，P<0.05], and promoted the protein expression of caspase 3 and PARP in NK-92 cells. Conclusion: miR-150 expression significantly decreased in both NK/T cell lymphoms tissues and cell lines. The patients of low miR-150 expression had low CR after radiotherapy. miR-150 mimics transfection promoted radiation-induced apoptosis of NK-92 cells. miR-150 has an enhancement effect on radio-sensitivity of NK/T cell lynphoma.
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Project Supported:
Project supported by the National Natural Science Foundation for Youth Scholar of China(No.81400156)
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