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Archive > Volume 24 Issue 8 > 2017,24(8):851-855. DOI:10.3872/j.issn.1007-385X.2017.08.006 Prev Next
Basic Research
The cytotoxicity of MDSC-derived from BaF3-RAE1ε injected mice and its effect on NK cell function
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Abstract:
Objective: To study the cytolytic activity of myeloid-derived suppressor cell (MDSC) from BaF3-RAE1ε injected mice and its effect on the function of NK cell. Methods: Two derivatives of murine pro-B cell line BaF3 cells, expressing empty plasmid (termed BaF3-mock) or retinoic acid early transcript 1ε (RAE1ε, termed BaF3-RAE1ε) were respectively constructed in the previous study. CD11b + Gr-1 + MDSC magnetically purified from BaF3-mock or BaF3-RAE1ε bearing mice were co-cultured with NK cells. After 24 hours, the cells were harvested for detecting NKG2D and CD107a expression on NK cells by flow cytometry, and the supernatants were collected for detecting IFN-γ by ELISA. Moreover, MDSC sorted from BaF3-mock or BaF3-RAE1ε bearing mice were co-cultured with BaF3-mock or BaF3- RAE1ε target cells. After 5 h, the cytotoxicity of MDSC against BaF3-mock or BaF3-RAE1 target cells was evaluated by the lactate dehydrogenase release test. Results: There was no obvious dif-ference in secretion of IFN-γ by NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05). No difference in NKG2D and CD107a expression was detected among NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05). MDSC isolated from BaF3-RAE1ε bearing mice had higher direct cytolytic activity against BaF3-mock or BaF3-RAE1ε cells than MDSC isolated from BaF3-mock bear-ing mice (P<0.01). Conclusion: RAE1ε enhances the cytolytic activity of MDSC against BaF3-mock or BaF3-RAE1ε cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81373130，No.81001308) ，the Natural Science Foun-dation of Jiangsu Province (No. BK2010315)，and the Science and Technology Innovation Foundation for Undergraduates of Yangzhou University
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