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Archive > Volume 24 Issue 8 > 2017,24(8):856-863. DOI:10.3872/j.issn.1007-385X.2017.08.007 Prev Next
Basic Research
Differentiation-inducing effect of MDA-7/IL-24 on Burkitt lymphoma cells
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Abstract:
Objective: To investigate the differentiation- inducing effect of melanoma differentiation associated gene 7（MDA-7）/IL-24 on human Burkitt lymphoma cells, as well as its underlying mechanism. Methods: Human Burkitt lymphoma cell lines (Raji and Daudi) that stably over-expressing MDA-7/IL-24 were constructed. The effect of MDA-7/IL-24 transfection on cell viability of Raji and Daudi cells was assayed by MTS method; the effect on mi-gration and invasion of Raji and Daudi cells were analyzed by Transwell assay. The apoptosis and immunopheno-types of Raji and Daudi cells with MDA-7/IL-24 over-expression were analyzed by flow cytometry. Meanwhile, the expressions of differentiation related proteins (Myb, BLIMP1 and BCL-6) were analyzed by Western blotting. Raji cell xenograft model was established on nude mice to analyze the effect of MDA-7/IL-24 on the bioactivity of Raji cells in vivo. Results: The cell viability, migration and invasion ability of Raji and Daudi cell lines that transfected with MDA-7/IL-24 were obviously decreased (all P<0.01), however, the apoptosis cells were not increased (P>0.05). The expressions of CD45 and CD138 were elevated (P<0.01) while the expression of CD10 was decreased (P<0.01) in Raji and Daudi cells overexpressing MDA-7/IL-24. MDA-7/IL-24 over-expression in Raji and Daudi cells significantly increased the expression of BLIMP1 (P<0.01), but decreased the expression of Myb and BCL-6(P＜0.01). The tumor mass of nude mice was significantly decreased after the treatment of MDA-7/IL-24 ([1.23±0.21] vs [1.96±0.24]g, P<0.01). Conclusion: Transfection with MDA-7/IL-24 inhibited the bio-actively of Burkitt lymphoma cells, which might be accomplished by inducing differentiation.
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Project Supported:
Project supported by the Medical Scientific Research Key Program of Hebei Prov-ince (No.20160171)
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