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Archive > Volume 24 Issue 8 > 2017,24(8):864-869. DOI:10.3872/j.issn.1007-385X.2017.08.008 Prev Next
Basic Research
miR-186 suppresses the proliferation and invasion and promotes apoptosis of os-teosarcoma cells partially through targeting PTTG1
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Abstract:
Objective: To explore the effects of miR-186 on the cell proliferation, apoptosis and invasion of hu-man osteosarcoma cells, and identify its putative mechanisms. Methods: The expression of miR-186 in osteosarco-ma cell lines (HOS, U2-OS and Saos-2) and osteoblast NHOst cells was detected using RT-PCR assays. Artificially synthesized miR-186 mimic and relative control scramble mimic was transfected into osteosarcoma HOS and U2-OS cell lines, and the expression of miR-186 in OS cells was detected using the RT-PCR assays upon transfection.Then, the effects of miR-186 over-expression on cell proliferation, apoptosis and invasion of OS cells were explored using the CCK-8, FCSE and transwell invasion assays, respectively. The effects of miR-186 over-expression on mRNAand protein expression of PTTG1 (pituitary tumor transforming gene1) were explored using the Western blot-ting and RT-PCR assays. Results: miR-186 was low expressed in OS cell lines; Transfection with artificially synthe-sized miR-186 mimic significantly up-regulated the expression of miR-186 in HOS and U2-OS cells; the prolifera-tion rate of cells transfected with miR-186 mimic was much lower than those transfected with scramble mimic [HOS: (16.9±2.1)% vs (10.4±1.6)%; U2-OS: (22.6±2.9)% vs (14.1±2.2)%; (P<0.01); the apoptotic rates of HOS and U2-OS cells transfected with miR-186 mimic were higher than those transfected with scramble mimic [HOS: (16.9±2.1)% vs (10.4±1.6)%; U2-OS: (22.6±2.9)% vs (14.1±2.2)%; all P<0.05], and the number of cells passing through the chambers in miR-186 mimic transfection group was less than those of scramble transfection group (P<0.01).The expression levels of PTTG1 at protein and mRNA level were both suppressed in cells transfected with miR-186 mimic (P<0.01); however, scramble transfection group showed no statistical difference (P>0.05). Conclusion: Over-expression of miR-186 significantly inhibited cell proliferation and invasion, and promoted the apoptosis of OS cells, which might be related with PTTG1 suppression.
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Project Supported:
Project sup-ported by the National Natural Science Foundation of China（No. 81502329）, and the Foundation of the Yongchuan Hospital of Chongqing Medical Uni-versity Grants (No. YCZQN201514).
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