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Archive > Volume 24 Issue 9 > 2017,24(9):938-943. DOI:10.3872/j.issn.1007-385X.2017.09.002 Prev Next
Prompt Report
Cytotoxicity of specific CTL induced by EBV-LMP2A peptide on human EBV associated gastric carcinoma cell in vitro
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Abstract:
Objective: To study the cytotoxicity of specific cytotoxic T lymphocyte (CTL) induced by EBV-LPM2A peptide on human EBV associated gastric cancer cells in vitro. Methods: The peripheral blood mononucle-ar cells (PBMC) of HLA-A2 positive gastric cancer patients from Cancer Center of Drum Tower Hospital Affiliated to Medical School of Nanjing University, human gastric adenocarcinoma cell line (AGS) and human EBV positive adenocarcinoma cell line (AGS-EBV) were selected. By modified in vitro cell culture technique, specific CTL was amplified from human PBMCs with the induction of HLA-A2 restricted EBV-LMP2A peptide; the content of specif-ic CTL induced by peptide was detected by the method of tetramer and Flow cytometry; the in vitro cytotoxicity of EBV-CTL on both EBV + and EBV - human gastric cancer cells was detected by FITC-PI. Results: Modified cell cul-ture technique and EBV-LMP2A peptide can induce a high proportion of antigen specific CTL (EBV-LMP2A-356 specific T cells accounted for [47.1±5.2]% of CD8 + T cells）; the cytotoxicity of EBV-CTL on EBV + gastric cancer cells was significantly stronger than that of EBV - gastric cancer cells ([45.1±9.3]% vs [19.4±2.5]%，P<0.05). Con-clusion: Using the modified cell culture technique, EBV-LMP2A antigen peptide can induce a high proportion of EBV specific CTL, which has high specific cytotoxicity on EBV + gastric cancer cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81602077，No. 81572601)
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