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Archive > Volume 24 Issue 9 > 2017,24(9):972-978. DOI:10.3872/j.issn.1007-385X.2017.09.008 Prev Next
Clinical Research
Expression and methylation status of miRNA-6803 and PPP6R1 in esophageal squamous cell carcinoma and their relationship with clinicopathological charac-teristics of ESCC patients
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Abstract:
Objective: To investigate the expression of microRNA-6803(miR-6803) and its host gene PPP6R1 in esophageal squamous cell carcinoma (ESCC) and the role of promoter methylation status of PPP6R1 in the tumori-genisis and progression of ESCC. Methods: ESCC tissues and corresponding para-cancerous tissues from 72 cases of ESCC patients stored in the bio-specimen base of the Fourth Hospital Affiliated to Heibei Medical University dur-ing 2013 and 2014 were collected for this study.Real-time fluorescence quantitative PCR (qPCR) was used to detect the expression of miR- 6803/PPP6R1 in collected tissues and in ESCC cell lines (TE1, TE13, Eca109, T.TN,Kyse170) treated or untreated with DNA methyl-transferase inhibitor 5-aza-2’-detoxycytidine (5-Aza-dC). Methyla-tion specific PCR (MSP) method was used to detect the methylation status of PPP6R1 in esophageal cancer cell lines and collected tissues, respectively. Results: The relative expressions of miR-6803 and PPP6R1 in ESCC tis-sues were significantly reduced compared to correspondingtissues (0.318±0.156, 0.408±0.177 vs 1.000±0.001, all P<0.05), and expression of miR-6803 was associated with lymph node metastasis, TNM stage and pathological differ-entiation (all P<0.05); The expression of PPP6R1 was associated with TNM stage and pathological differentiation (all P<0.05). There was a significant correlation between the relative expression of miR-6803 and PPP6R1 in ESCC tissues (P<0.05).The promoter methylation frequency of PPP6R1 in ESCC tissues was significantly higher than that in corresponding normal tissues (56.94% vs 36.11%, P<0.05), and was also associated with TNM stage and patho-logical differentiation (all P<0.05). The low expression of miR-6803/PPP6R1 was significantly correlated with pro-moter methylation of PPP6R1 in ESCC tissues (P<0.05). The relative expression level of miR-6803 and PPP6R1 in 5 ESCC cell lines was enhanced after the treatment with 5-Aza-dC. After treatment with 5-Aza-dC, the methylation level of PPP6R1 was decreased in TE1, TE13, Kyse170 cells, while complete unmethylation of PPP6R1 was detect-ed in other two cell lines. Conclusion: Aberrant low expression of miR-6803 and its host gene PPP6R1 are closely related to the occurrence and development of ESCC, and promoter methylation may be one of the mechanisms for inactivation of miR-6803/PPP6R1 in ESCC.
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Project Supported:
Projects supported by the National Natural Science Foundation of China（No. 81572441）, and the Key Project of Medical Science Research of Hebei Province（No.20170700，No.20170701）
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