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Archive > Volume 24 Issue 9 > 2017,24(9):984-989. DOI:10.3872/j.issn.1007-385X.2017.09.010 Prev Next
Clinical Research
Effect of expression of Testin gene in non-small cell lung cancer tissues and cell lines on the malignant biological beharior of cancer cells
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Abstract:
Objective: To investigate the expression of Testin (TES) gene in non-small lung cancer cell (NSCLC) lines, and also to investigate its effect on cell proliferation, migration, invasion and apoptosis of human lung cancer cell line A549. Methods: Twenty-seven cases of cancer tissues and corresponding para-cancerous tissues from non-small lung cancer patients that treated in Tongji Hospital Affiliated to Tonji Medical College of Huazhong Universi-ty of Science and Technology between January 2015 and December 2015 were collected for this study; and the ex-pression of TES protein in collected tissues was examined by Western blotting. In the meanwhile,TES protein ex-pressions in six human lung cancer cell lines (A427, A549, H1299, LK2, PC9 and SW900) and lung fiberblast cell line MRC5 were also detected. shTES was transiently transfected into A549 cells to interfere the expression of TES gene; the effect of low TES expression on proliferation, migration, invasion and apoptosis of A549 were further as-sessed, and its effect on the expressions of apoptosis related proteins (Bax, Bcl-2 and Caspase-3) were also tested by Western blotting. Results: The expression of TES protein in lung cancer tissues and lung cancer cell lines was signif-icantly decreased (all P<0.05). After shTES transfection in A549 cells, the expressions of TES mRNA and protein were significantly down-regulated (P<0.05). Additionally, shTES significantly increased cell proliferation ([2.75±0.04］vs [1.79±0.06], P<0.05), migration ([52.3±2.6]% vs [19.7±1.4]%, P<0.05), and invasion ([31.2±3.89]% vs [14.5±4.1]%, P<0.05), while inhibited cell apoptosis ([8.2±1.1]% vs [23.1±1.7]%, P<0.05) of A549 cells. Moreover,TES low expression significantly decreased the expressions of Bax and Caspase-3 (P<0.05) but increased the expres-sion of Bcl-2 (P<0.05) in A549 cells. Conclusion:TES was low expressed in NSCLC tissues, and down-regulation of TES could promote cell proliferation, migration, invasion and inhibit the apoptosis of lung cancer cells, which may serve as a new target for lung cancer treatment.
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