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Archive > Volume 24 Issue 9 > 2017,24(9):995-1001. DOI:10.3872/j.issn.1007-385X.2017.09.012 Prev Next
Clinical Research
Expression of glutathione peroxidase-1 in colorectal cancer tissue and its effect on cell proliferation, invasion and migration of cancer cells
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Abstract:
Objective: To investigate the expression level of glutathione peroxidase 1（GPX1）in colorectal can-cer (CRC) tissues, and its effect on the proliferation and migration of CRC HT29 and LOVO cell lines. Methods:Colorectal cancer tissues and para-cancerous tissues that resected from 60 CRC patients at Binjiang Branch of Haik-ou People’s Hospital from June, 2015 to March, 2016 were collected for this study. Real-time fluorescence quantita-tive PCR was used to detect the expression level of GPX1 mRNA in sample tissues. HT29 cell line expressing high-er GPX1 mRNA was transfected with lentivirus shRNA to stably knock-down GPX1 expression, while LOVO cell line expressing lower GPX1 mRNA was subjected to transient transfection method to stably overexpressing GPX1,and the protein and mRNA expression level of GPX1 were verified in cells. The change in cell proliferation ability was detected by MTS assay, and the invasion and migration ability was detected by Transwell invasion assay and wound healing assay. The changes in expression of E-cadherin and vimentin were detected by Western blotting.Results: GPX1 mRNA expression was significantly lower in CRC tissues compared with para-cancerous tissues (0.051±0.044 vs 0.142±0.051, P<0.01). After knock-down of GPX1: the proliferation ability of HT29 cells was sig-nificantly improved (12.901±2.790 vs 6.617±2.462, P<0.01), the invasion and migration ability of HT29 cells were significantly enhanced (invasion: [384.7±37.9] vs [209.2± 31.2], P<0.01; migration: [0.139±0.025] mm vs [0.251±0.038] mm, P<0.01); and there was a down-regulation of E-cadherin (P<0.01) and an up-regulation of vimentin (P<0.01). After overexpression of GPX1: the proliferation ability of LOVO cell was significantly decreased (P<0.01);the invasion and migration ability of LOVO cell was significantly decreased (all P<0.01); and there was an up-regu-lation of E-cadherin (P<0.01) and a down-regulation of vimentin (P<0.01). Conclusion: GPX1 mRNA was low-ex-pressed in human colorectal cancer tissues. GPX1 may inhibit the proliferation, invasion and migration of CRC cell lines, and may play an important anti-carcinoma role in CRC.
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