Mobile website
Archive > Volume 24 Issue 10 > 2017,24(10):1058-1062. DOI:10.3872/j.issn.1007-385X.2017.10.003 Prev Next
Prompt Report
Expression characteristics of adhesion molecules on HLCMVEC and its influence on adhesion and trans-endothelial migration of PBMC
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the expression characteristics of adhesion molecules on human liver cancer microvascular endothelial cells (HLCMVECs) and its influence on adhesion and trans-endothelial migration of im-mune cells. Methods: HLCMVECs were isolated and cultured, and human liver sinusoid endothelial cells (LSECs) were used as control. The expression of adhesion molecules was evaluated by cell-based ELISA. Peripheral blood mononuclear cells (PBMCs) were labeled with BCECF (a fluorescent dye) and then co-cultured with HLCMVEC or LSEC; the adhesion to endothelial cells and trans-endothelial migration of PBMCs were observed by inverted fluo-rescence microscope and continuous spectrum luminoscope. In addition, the functional antibody against each adhe-sion molecule was respectively added to endothelial cells before co-culture, which could determine the contribution of each adhesion molecule to the adhesion and trans-endothelial migration of PBMC. Results: Compared to LSECs,HLCMVECs expressed low level of CD31, CD34 and intercellular adhesion molecule-3 (ICAM-3) (P<0.05) and an even lower level of intercellular adhesion molecule-1 (ICAM-1) and Vascular cell adhesion molecule-1 (VCAM-1)(P<0.01), but expressed significantly higher level of αvβ3 and αvβ5 (P<0.01). Compared with LSECs, the adhesion of PBMCs to HLVMVECs were significantly decreased; and under the inducement of chemo-tactic agent, trans-en-dothelial migration of PBMCs through HLVMVECs were also significantly decreased (adhesion: [205.5±46.0] vs [330.5±48.4]; migration: [49.0±10.6] vs [110.0±19.2]; all P<0.01). However, the adhesion and migration could be obviously blocked by antibodies against ICAM-3 (P<0.05), ICAM-1 and VCAM-1(P<0.01); Antibody against CD31 did not influence PBMC adhesion greatly but significantly reduced trans-endothelial migration (P<0.05).Conclusion: The distinct expression of adhesion molecules on HLCMVECs reduced the adhesion and trans-endo-thelial migration of PBMCs.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81402451，No. 81370873), and the Major National Ba-sic Research Development Program (973 Program) of China (No. 2009CB521804)
Clc Number:
