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Archive > Volume 24 Issue 10 > 2017,24(10):1088-1092. DOI:10.3872/j.issn.1007-385X.2017.10.008 Prev Next
Basic Research
Effect of bone marrow mesenchymal stem cells transfected with ADAM17-shRNA on growth of implanted breast cancer in nude mouse
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Abstract:
Objective:To investigate the inhibitory effect of bone marrow mesenehymal stem cells (BMMSCs)transfected with ADAM17-shRNA (adisintegrin and metalloprotease 17-shRNA) on the growth of implanted breast cancer MCF-7 cell xenograft in nude mice. Methods: BMMSCs from 3-week-old male SD rats were isolated and cultured with the whole bone marrow adherence method. BMMSCs were transfected with Lentivirus-mediated AD-AM17-shRNA. Breast cancer MCF-7 cell xenograft model was successfully established in 30 nude mice after 14 days implantation of tumor cells.According to the random number table, nude mice were randomly divided into con-trol group (equal volume PBS), BMMSCs group (1×10 6 /ml BMMSCs) and transfection group (1×10 6 /ml BMMSCs transfected with ADAM17-shRNA) with 10 nude mouses in each group. The tumor inhibition test was carried out on the 15 th day by injecting BMMSCs into tail vein (0.1 ml/each, administration was carried every 3 days with a to-tal of 5 times). The growth of implanted tumor was observed every day. All the nude mice were sacrificed on 16 th day after treatment. The expressions of ADAM17 mRNA and ADAM17 protein in tumor tissues were detected by Real- time PCR and Western blotting, respectively. Results：The volume of implanted tumor in control group,BMMSCs group was significantly larger than that of transfection group ([787.15 ± 25.95], [767.02 ± 28.98] vs [361.89±19.75] mm 3 , all P<0.01) on D 30. The tumor inhibition rate of BMMSCs group and transfection group was significantly higher than that of control group (2.57%, 53.89% vs 0.00%, all P<0.05). The expression of ADAM17 mRNA in control group , BMMSCs group was significantly higher than that of transfection group (1.00±0.01, 0.97±0.08 vs 0.30±0.09, P<0.05). The expression of ADAM17 protein in control group, BMMSCs group was significant-ly higher than that of transfection group (0.70±0.09, 0.68±0.02 vs 0.45±0.05, all P<0.05). Conclusion: The tropism of ADAM17-shRNA to breast cancer xenograft in nude mice was accomplished by BMMSCs mediation, which may play an anti-tumor effect.
Keywords:
adisintegrin and metalloprotease 17-shRNA (ADAM17-shRNA) ; breast cancer ; MCF-7 cell ; bone marrow mesenehymal stem cell (BMMSC)
Project Supported:
Project supported by Tangshan City Science and Technology Research and Development Foundation （No.14130256B）
