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Archive > Volume 24 Issue 11 > 2017,24(11):1271-1275. DOI:10.3872/j.issn.1007-385X.2017.11.005 Prev Next
Basic Research
Effects of HuR siRNA on proliferation, migration and invasion of the lung adenocarcinoma A549 cells
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Abstract:
Objective: To explore effect of HuR siRNA on proliferation, migration and invasion of the human lung adenocarcinoma cell line A549 and its possible mechanism. Methods: After the A549 cells were transfected by HuR siRNA and cultured for 24 hours, CCK-8 assay and clone formation assay were used to detect proliferation ability of the A549 cells. Migration and invasion abilities of the A549 cells were respectively detected by scratch and Transwell assays. mRNA and protein expressions of HuR and matrix metalloproteinose-9 (MMP-9) were detected by RTqPCR and Western blotting assays. Results: HuR siRNA inhibited proliferation, migration and invasion of the human lung cancer cell line A549. HuR siRNA significantly reduced mRNA and protein expressions of HuR and MMP-9 in the A549 cells (P<0.01). Conclusion: Down-regulation of HuR could inhibit proliferation, migration and invasion of the lung adenocarcinoma A549 cells, and the inhibitions of migration and invasion of the A549 cells might be related to down-regulation of MMP-9.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81572875，81272619）
Clc Number:
