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Archive > Volume 24 Issue 11 > 2017,24(11):1276-1280. DOI:10.3872/j.issn.1007-385X.2017.11.006 Prev Next
Basic Research
Sodium tanshinone IIA sulfonate inhibits migration of the cervix carcinoma HeLa cell via down-regulating expression of integrin β3
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Abstract:
Objective: To explore effect of sodium tanshinone IIA sulfonate (STS) on migration of the cervix carcinoma Hela cell and its possible mechanism. Methods：Effects of STS in different concentrations, specific antibody to integrin β3 (LM609), and transfection with integrin β3 over-expression plasmid on migration of the cervix carcinoma HeLa cell were detected by Transwell assay. Immunoblotting test was used to detect effects of STS in different concentrations and different times of STS stimulation on expression of integrin β3 in the HeLa cell. Effect of STS on viability of the HeLa cell was tested by CCK-8 assay. Results：STS in concentration-dependent manner reduced significantly migration of the HeLa cell (P<0.01). LM609 remarkably decreased migration rate of the HeLa cell (P<0.01). Migration rate of the HeLa cell transfected with over-expression integrin β3 plasmid and stimulated by STS was markedly more increase than those of the HeLa cell stimulated by STS only as well as the HeLa cell transfected with empty plasmid and stimulated by STS (P<0.01). STS in concentration- and timing-dependent manners reduced expression of integrin β3 in the HeLa cell (P<0.05). There was no significant difference in number of the viable cells between the HeLa cell stimulated with different concentrations of STS groups and the HeLa cells in blank control group (P>0.05). Conclusion：STS could reduce expression of integrin β3 in the cervix carcinoma HeLa cell, and significantly decrease migration of the HeLa cell，LM609 which is a specific antibody against integrin β3 could also reduced migration of the HeLa cells, and transfection with integrin β3 over- expression plasmid could reduce the inhibition of STS on migration of the HeLa cell. The results of this paper shown that STS might inhibits migration of the HeLa cell via decrease of integrin β3 expression, which might provide theoretical basis and experimental evidence for further explain molecular mechanism of STS anti-carcinoma and clinical application of STS.
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