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Archive > Volume 24 Issue 11 > 2017,24(11):1293-1298. DOI:10.3872/j.issn.1007-385X.2017.11.009 Prev Next
Clinical Research
Expression of periostin in the mammary cancer and its clinical significance
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Abstract:
Objective: To explore expression status of periostin (POSTN) in the mammary cancer, para-cancer and normal mammary tissues and its clinical significance. Methods: The mammary cancer, para- cancer and normal mammary tissues of 45 patients with mammary cancer，which were taken out by exairesis in Department of Galactophore,Third Affiliated Hospital of Kunming Medical University during May 2012 to December 2016. In addition,from the tissues fifteen amicrobic mammary cancer and normal mammary tissues were selected for primary cell culture.The primary cultured cells were identified by HE staining and immunohistochemistry assays. Expression status of POSTN mRNA in the mammary cancer, para-cancer and normal mammary tissues as well as in the cancer associated fibroblast (CAF) and normal fibroblast (NF) were detected by real-time quality PCR (qPCR). Correlation analysis was used to analyze the relationship between expression level of POSTN in the mammary tissue and clinical pathological features of the patients with mammary cancer. Location of POSTN protein in the mammary cancer tissue was identified by HE staining and immunohistochemistry of the mammary cancer tissue chips. Results: Expression level of POSTN mRNA in the mammary cancer tissue was significantly higher than those in the para-cancer and normal mammary tissues (P<0.05), expression level of POSTN mRNA was similar between CAF and NF (P>0.05).POSTN protein mainly expressed in substances of the mammary cancer tissues and less expressed in the mesenchyma of the mammary cancer tissue. Expression level of POSTN mRNA in the mammary cancer tissue was negatively correlated to estrogen receptor (ER) of the patients with mammary cancer (P<0.05). Conclusion: POSTN was mainly expressed in substances of the mammary cancer tissues, high expression of which in the mammary tissues could be related to occurrence and development of the mammary cancer.
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Project Supported:
Project supported by the National Natural Science Foundation（No.81460358）, the Joint Special Program of Yunnan Province Department and Kunming Medical University for applied basic research [No.2017FE467(- 189)，2014FB066], and the Science and Technology Planning Project of Yunnan Provincial Health and Family Planning Commission（No.2017NS201, 2014NS023）
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