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Archive > Volume 24 Issue 12 > 2017,24(12):1386-1390. DOI:10.3872/j.issn.1007-385X.2017.12.008 Prev Next
Basic Research
Colony stimulating factor-1 receptor-mediated Bax/Bcl-2 expression inhibits apoptosis of human nasopharyngeal carcinoma 6-10B cells
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Abstract:
Objective: To investigate the inhibitory effect of Colony stimulating factor- 1 receptor (CSF- 1R) on apoptosis of human nasopharyngeal carcinoma 6-10B cells and its relationship with Bax/Bcl-2 expression. Methods:Lentiviral vector over- expressing CSF-1R LV-CSF1R（16957-1）was constructed and trasfected into 6-10B cells; meanwhile a control group was set; The expression of CSF-1R, Bcl-2 and Bax was detected by Real-time PCR and Western blotting. CCK-8 assay and flow cytometry (FCM) were used to detect the cell proliferation and apoptosis,respectively. Results: The mRNA expression of CSF-1R increased significantly in 6-10B cells of transfection group compared with negative control (NC) group (7.01±0.23 vs 0.09±0.03, P<0.01); The expression of Bax mRNA was significantly down-regulated while the expression of Bcl-2 mRNA was significantly up-regulated in 6-10B cells of transfection group (all P<0.01). The protein expression of CSF-1 in 6-10B cells of transfection group was significantly higher than that of control group, along with the remarkably down- regulated Bax protein expression and slightly up-regulated Bcl-2 protein in transfection group. Compared with NC group, the proliferation vitality of 6-10B cells were extremely increased after transfection (P<0.01); however, the cell apoptosis was significantly reduced ([10.82±0.75]% vs [17.11±0.46]%, P<0.05). Conclusion: Over-expression of CSF-1R could promote the ma-lignant growth and inhibit the apoptosis of nasopharyngeal carcinoma 6-10B cells by regulating the ratio of Bax/Bcl-2 expression.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81260348）
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