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dsP21-625 inhibits the proliferation of prostate cancer cells by activating P21 gene expression
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Abstract:
Objective: To study the effects of synthetic small molecule double-stranded RNA-625（dsP21-625）on the activation of P21 gene in prostate cancer cells and its effect on cell proliferation. Methods: dsCtrl (control group) and dsP21-625（experimental group）were transfected into prostate cancer PC-3 and DU-145 cell lines. qPCR and Western blotting were used to detect the mRNA and protein expressions of P21, Cyclin E and cyclin dependent kinase 2（CDK2）in prostate cancer cells of each group after transfection. The cell cycle distribution, cell proliferation and clone formation were analyzed by flow cytometry, MTT assay and colony formation assay,respectively. Results: Compared with dsCtrl control group, P21 mRNA level was elevated in PC-3 cells and in DU-145 cells (all P<0.01) after transfection with dsP21-625; in the meanwhile, the expression of Cyclin E and CDK2 mRNA were down-regulated (P<0.01).The expression of P21 protein in PC-3 and DU-145 cells transfected with dsP21-625 was up-regulated (all P<0.01) while the expressions of Cyclin E and CDK2 proteins were down-regulated (all P<0.01); the proportion of cells in S phase and G2 / M phase decreased (all P<0.05), and the proportion of cells in G0/G1 phase increased (all P<0.01) after dsP21-625 transfection. The cell proliferation ability and colony formation were significantly decreased in dsP21-625 groups (all P<0.05). Conclusion: dsP21-625 can activate the expression of P21 mRNA and protein in prostate cancer cells, down-regulate the expression of Cyclin E and CDK2 protein, and significantly inhibit the proliferation of prostate cancer cells.
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Project Supported:
Project supported by the Natural Science Foundation of Hubei Province（No.2017CFB176）
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