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Expression of miR- 372- 3p in the tissues of bladder carcinoma and its effect on proliferation, migration and invasion of the bladder cancer cells
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Abstract:
Objective: To detect expression of miR-372-3p in the bladder cancer cell transfected with miR-372-3p mimics and to explore effect of miR-372-3p on proliferation, migration and invasion of the bladder cancer 5637 and T24 cell lines. Methods: Using lipofection,miR-372-3p mimics or miR-NC was transfected into the bladder cancer 5637 and T24 cells. Expressions of miR-372-3p mRNA and ATAD2 mRNA, expressions of ADAD2, E-cadherin and N-cadherin proteins, cell cycle distribution, viability of cell, cell proliferation ability, cell migration ability and cell invasion ability in the bladder cancer cells transfected with miR-372-3p mimics and miR-NC were respectively detected by qPCR, Western blotting assay, flow cytometry, MTT assay, colony forming test, scratch assay and Transwell assay. Results: Comparing with the bladder cancer cell transfected with miR-NC, expression of miR-372-3p mRNA significantly increased, expressions of ATAD2 mRNA and protein obviously decreased, expression of E-cadherin protein raised, expression of N-cadherin protein came down, cell cycle obviously arrested, abilities of cell viability and proliferation markedly reduced as well as cell numbers of migration and invasion reduced, in the bladder cancer cell transfected with miR-372-3p mimics. Conclusion: Low expression of miR-372-3p might be associated with occurrence and development of the bladder carcinoma. miR-372-3p could inhibit abilities of proliferation,migration and invasion of the bladder cancer cell through targeting control. It could be a novel target in biotherapy of the bladder carcinoma.
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