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Recombinant protein PTD-CHMP1A inhibits malignant biological behavior of renal cell carcinoma cells
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Abstract:
Objective: To study the influence of PTD-CHMP1A (protein transduction domain-chromatin modifying protein 1A) fusion proteins on proliferation, migration and invasion of renal cell carcinoma (RCC) cells in vitro and the growth of transplanted tumor in vivo.Methods: RCC A498 and 786-0 cells were treated with PTD-CHMP1A (1, 5, and 20 μg/ml), CHMP1A (5 μg/ml) and PBS respectively.The effect of PTD-CHMP1A on the proliferation of A498 cells was detected by MTT assay. The effect of PTD-CHMP1A on the migration of A498 cells was detected by scratch assay. The effect of PTD-CHMP1A on the invasion ability of A498 and 786-0 cells was detected by Transwell invasion assay. Finally, the transplanted tumor models were constructed by injecting 5×105 A498 cells into nude mice, and the effect of 20 μg PTD-CHMP1A on tumor growth was observed. Results: Compared with CHMP1A and PBS groups, PTDCHMP1A fusion proteins (1, 5, and 20 μg/ml) showed a significantly restrained effect on the proliferation of A498 tumor cells, which was in dose-dependent and time-dependent manners. 5 μg/ml of PTD-CHMP1A fusion proteins could effectively inhibit the migration of A498 cells (P<0.01 compared with CHMP1A group), and the invasion of A498 and 786-0 cells (compared with Chmp1A group, P<0.05), besides, PTD-CHMP1A inhibited the invasion of A498 cells in a dose-dependent manner. Furthermore, PTD-CHMP1A could also observably inhibit tumor growth in vivo. Conclusion: PTD-CHMP1A fusion proteins effectively inhibited the proliferation, migration and invasion of RCC cells and the growth of transplanted tumor.
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Project Supported:
Project supported by the Natural Sciences Foundation of Zhejiang (No. QY18H160001, No. LY13H160031)
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