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MiR-129-5p influences the sensitivity of breast cancer MCF-7 cells to paclitaxel by regulating HMGB1
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Abstract:
Objective: To investigate the effect of miR-129-5p on the sensitivity of breast cancer MCF-7 cells to paclitaxel (PTX) by regulating high mobility group box 1 (HMGB1). Methods: MCF-7 cells were transfected with miR-129-5p mimics or si-HMGB1 by liposomes transfection technology before stimulated with PTX, respectively. Then, the mRNA expressions of HMGB1 and miR-129-5p in MCF-7 cells were detected by Real-time fluorescence quantitative PCR. Assessment of the expression of HMGB1 protein in MCF-7 cells was performed using Western blotting, and the effect of PTX on the proliferation of MCF-7 cells was performed by CCK-8 assay.Finally, the effect of transfection on PTX-induced apoptosis of MCF-7 cells was detected by flow cytometry. Results: After being transfected with miR-129-5p mimics, the expression of miR-129-5p was significantly higher than that of the negative control group (P<0.01). Over-expression of miR-129-5p significantly enhanced the inhibition of MCF-7 cells proliferation by PTX (P< 0.05) and PTX-induced apoptosis (P<0.05), meanwhile it also significantly inhibited the xpression of HMGB1 mRNA and protein (all P<0.05). Transfection with si-HMGB1 significantly reduced the expression of HMGB1 mRNA and protein (all P<0.05) in MCF-7 cells. HMGB1 interference further promoted PTX-induced proliferation inhibition and apoptosis of MCF-7 cells (all P<0.05). Conclusion: miR-129-5p enhanced the sensitivity of MCF-7 cells to PTX by down-regulating HMGB1.
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Project Supported:
Project supported by the Key Research Program of Higher Education in Henan Province（No.14A320038）
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