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Long chain non-coding MALAT1 affects biological behavior of pancreatic cancer cells by regulating miR-204 expression
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Abstract:
Objective: To investigate the effect of long-chain non-coding RNA metastasis-associated lung adenocarcinoma transcript 1（lncRNA MALAT1）on the malignant biological behavior of pancreatic cancer cells by regulating miR-204 expression and its possible mechanisms. Methods: Six pairs of pancreatic cancer tissues and corresponding para-cancerous tissues were sampled during surgery from October 2016 to December 2016 in Henan Provincial Hospital of Traditional Chinese Medicine. qPCR was used to detect the expression of lncRNA MALAT1 in pancreatic cancer tissues, adjacent normal tissues and different pancreatic cancer cells（BxPC-3，HS-7667，PANC-1，AsPC-1 and SW-1990). Double luciferase reporter gene assay was used to detect the interaction between MALAT1 and miR-204. Flow cytometry was used to determine the effect of MALAT1 on cell cycle and apoptosis of pancreatic cancer cells. The effects of MALAT1 and miR-204 on the migration and invasion of pancreatic cancer cells were examined by Wound-healing assay and Transwell invasion assay, respectively. The effect of MALAT1 on EMT-related proteins was detected by Western blotting. Results:Compared with para-cancer tissue, the expression of lncRNA MALAT1 in pancreatic cancer tissues was significantly increased (1.85±0.52 vs 0.34±0.12，P<0.05). The expression level of lncRNA MALAT1 in pancreatic cancer SW-1990 cell line was the highest (P<0.05). lncRNA MALAT1 could bind specifically to the 3 'UTR of miR- 204 to modulate the expression of miR- 204. Inhibition of MALAT1 expression could induce G2/M cell cycle arrest, and thus promote SW-1990 cell apoptosis; moreover, the ability of migration and invasion of SW-1990 cells was weakened and EMT-related proteins (N-cadherin, E-adherin and vimentin) were down-regulated after inhibiting the expression of MALAT1. However, over-expression of miR-204 could promote the migration and invasion of SW-1990 cells. Conclusion: lncRNA MALAT1 plays an important role in the development and progression of pancreatic cancer. It can regulate the malignant biological behavior of pancreatic cancer cells by targeting miR-204.
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Project Supported:
Project supported by the Science and Technology Breakthrough Project of Henan Province（No.142102310040）
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