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Archive > Volume 25 Issue 2 > 2018,25(2):153-157. DOI:10.3872/j.issn.1007-385X.2018.02.008 Prev Next
Clinical Research
Expression of plasma-cytoma variant translocation gene 1 in ovarian cancer and its malignant biologic behavior
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Abstract:
Objective: To investigate the effects of plasma-cytoma variant translocation gene 1 (PVT1) on proliferation, migration and invasion of ovarian cancer SKOV3 cells. Methods: Quantitative reverse transcription PCR (qRT-PCR) was used to detect the expression of PVT1 in 32 pairs of ovarian cancer tissues and corresponding para-carcinoma tissues. The effects of PVT1 on proliferation, migration and invasion of ovarian cancer cells were studied by CCK-8, scratch wound healing assay and Transwell assay. Results: The expression level of PVT1 in SKOV3 cells and ovarian cancer tissues was significantly increased (all P<0.01). The expression level of PVT1 was correlated with histological grade, FIGO stage and lymph node metastasis in patients with ovarian cancer (P<0.05 or P<0.01). After transfection with PVT1 siRNA for 36, 48 h, expression of PVT1 was significantly decreased in SKOV3 cells; and the inhibition of PVT1 expression could decrease the proliferation ability and inhibit the migration and invasion of SKOV3 cells (P<0.05 or 0.01). Conclusion: LncRNA PVT1 was highly expressed in ovarian cancer. Down-regulation of PVT1 could inhibit the proliferation,migration and invasion of ovarian cancer SKOV3 cells.
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Project Supported:
Project supported by the Scientific Program of Henan Science and Technology Department（No.162102310131）
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