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Archive > Volume 25 Issue 3 > 2018,25(3):236-239. DOI:10.3872/j.issn.1007-385X.2018.03.004 Prev Next
Basic Research
Suppressive effect of Tet-on mediated livin RNA interference on growth of lung carcinoma xenegraft in a rat model
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Abstract:
Objective: To investigate he effect of tetracycline- (Tet-on) mediated livin RNA interference on growth of lung carcinoma xenegrafts, and find a better regulatory way to interfere the development on lung cancer. Methods: livin shRNA lentiviral vectors were constructed; and the lung cancerA549 cells were subcutaneously injected into right upper back of nude mice to establish xenegraft mod- el. The livin shRNA lentiviral vectors were injected into xenografts to interfere the expression of livin, then tetracycline was injected in- traperitoneally for the induction. The suppressive effect of Tet-on mediated livin RNA interference efficiency was investigated and lung cancer xenograft development was observed. Results: After the induction with Tet-on, livin gene expression was significantly inhibited by livin shRNA compared with the control group and Tet-on-NC group; the xenograft volume in Tet-on- livin shRNA group was signifi- cantly smaller than that in control group and Tet-on-NC group ([5.31±0.86]g vs [8.22±0.63]g and [7.17±0.54] g, P<0.05). Moreover, lit- tle body toxicity was observed and no nude mice died in this study. Conclusion: The Tet-on mediated livin shRNA could suppress the growth of lung cancer development with good targeting and controllable characteristics, which might provide a potent tool for treating lung cancer with livin protein as target.
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Project Supported:
Project supported by the Natural Science Foundation of Fujian Province (No. 2015J01376), and the Foundation of Foujian Provincial Hospital for Ex- cellent Young Physicians (No. 2014YNQN03)
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