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Archive > Volume 25 Issue 3 > 2018,25(3):263-269. DOI:10.3872/j.issn.1007-385X.2018.03.009 Prev Next
Basic Research
The effect of FBXW7 gene point mutation to colorectal cancer HCT-116 cells’pro- liferation, migration, invasion, and its apoptosis resistance
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Abstract:
Objective: To investigate the impact of FBXW7 gene mutation on cell proliferation, apoptosis, migration, and invasion pro- cesses of colorectal cancer HCT-116 cell line. Methods: Recombinant plasmids carrying wild-type and mutant-type FBXW7 SNP were constructed and transfected into HCT-116 cell line; the FBXW7 protein expression level in HCT-116 strains after transfection was de- tected by Western blotting. Subsequently, cell proliferation capacity was tested by CCK-8 assay; tumor cell colony formation ability was tested by HTCA; cell apoptosis function was tested by FCM; cell migration and invasion were tested by scratch assay and Tran- swell assay, respectively. Results: Higher HBXW7 protein expression level was detected in HCT-116 strain transfected with wild-type HBXW7 in comparison to the control group (strains transfected with mutant-type HBXW7), negative-control (strains transfected with empty plasmids), and blank-control (strains untransfected) (all P<0.05). Compared with the other groups, strains transfected with wild- type HBXW7 exhibited significantly reduced proliferation, colony formation, migration and invasion ability (all P<0.05), but obviously increased apoptosis rate (P<0.05). Conclusion： ：FBXW7 gene mutation can down-regulate its protein expression, and further promote the proliferation, migration and invasion as well as inhibit the apoptosis of HCT-116 cells.
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Project Supported:
Project supported by the Natural Science Foundation of Chongqing Municipal- Science and Technology Commission (No. cstc2012jjA10038)
Clc Number:
